Kongmuang U, Luk J M, Lindberg A A
Department of Clinical Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.
J Clin Microbiol. 1994 Dec;32(12):3072-4. doi: 10.1128/jcm.32.12.3072-3074.1994.
Three different stool sample-processing methods (centrifugation, immunomagnetic separation, and selective enrichment cultivation) for the identification of Salmonella serogroups by PCR were studied. The corresponding sensitivities in an ethidium bromide stained-agarose gel were 10(5), 10(3), and 10 bacteria, respectively. The PCR assay with overnight enrichment performed as well as, or even better than, the conventional culture technique. Of 485 clinical stool samples, PCR correctly identified all 230 culture-positive samples as well as mixed Salmonella infections in four cases.
研究了三种不同的粪便样本处理方法（离心、免疫磁珠分离和选择性富集培养），用于通过PCR鉴定沙门氏菌血清型。在溴化乙锭染色的琼脂糖凝胶中，相应的灵敏度分别为10⁵、10³和10个细菌。过夜富集后的PCR检测效果与传统培养技术相当，甚至更好。在485份临床粪便样本中，PCR正确鉴定了所有230份培养阳性样本以及4例混合沙门氏菌感染病例。
