Rathod H, Malcolm A J, Gillespie J I, Berry V, Pooley J, Piggott N H, Datta H K
Department of Clinical Biochemistry, Medical School, University of Newcastle-upon Tyne, U.K.
J Pathol. 1994 Dec;174(4):293-9. doi: 10.1002/path.1711740409.
We report here a case of primary osteoclastoma that despite possessing HLA-DR-positive status and 'functional' calcitonin receptors, exhibited aggressive in vitro and in vivo bone resorptive activity. In the osteoclast bone slice assay employing scanning electron microscopy, the giant cell-mediated bone resorption was uninhibited by salmon calcitonin (10 nM) and significantly inhibited by raised extracellular calcium (20 mM). In Fura-2AM based microspectrofluorimetric assays, the presence of the 'functional' calcitonin receptors was ascertained by a rise in intracellular calcium induced by calcitonin and high extracellular calcium. These findings provide evidence for a hitherto unrecognized subtype of giant cells that have HLA-DR-positive status, exhibit avid bone resorptive activity, but remain insensitive to calcitonin despite possessing calcitonin receptors.
我们在此报告一例原发性破骨细胞瘤病例，该病例尽管具有HLA - DR阳性状态和“功能性”降钙素受体，但在体外和体内均表现出侵袭性的骨吸收活性。在采用扫描电子显微镜的破骨细胞骨切片试验中，巨细胞介导的骨吸收不受鲑鱼降钙素（10 nM）抑制，而细胞外钙升高（20 mM）则可显著抑制。在基于Fura - 2AM的显微荧光测定中，通过降钙素和高细胞外钙诱导的细胞内钙升高确定了“功能性”降钙素受体的存在。这些发现为一种迄今未被认识的巨细胞亚型提供了证据，该亚型具有HLA - DR阳性状态，表现出强烈的骨吸收活性，但尽管拥有降钙素受体，却对降钙素不敏感。
