采用EHEC-Tek酶联免疫吸附测定法检测肉类中的大肠杆菌O157:H7。
Detection of Escherichia coli O157:H7 in meat by an enzyme-linked immunosorbent assay, EHEC-Tek.
作者信息
Johnson R P, Durham R J, Johnson S T, MacDonald L A, Jeffrey S R, Butman B T
机构信息
Health of Animals Laboratory, Agriculture and Agri-Food Canada, Guelph, Ontario.
出版信息
Appl Environ Microbiol. 1995 Jan;61(1):386-8. doi: 10.1128/aem.61.1.386-388.1995.
Abstract
Investigation of the specificity of an enzyme-linked immunosorbent assay (ELISA) for detection of Escherichia coli O157:H7 in raw meats (EHEC-Tek; Organon Teknika Corp.) revealed that the target antigens of the detection reagent, monoclonal antibody 4E8C12, were present in numerous serotypes of E. coli and that their ELISA reactivity was influenced by bile salts, acriflavine, and heat. The specificity of the ELISA was improved by a modified test protocol incorporating immunocapture.
摘要
对用于检测生肉中大肠杆菌O157:H7的酶联免疫吸附测定（ELISA）（EHEC-Tek；Organon Teknika公司）的特异性进行的调查显示，检测试剂单克隆抗体4E8C12的靶抗原存在于多种大肠杆菌血清型中，并且它们的ELISA反应性受胆盐、吖啶黄素和加热的影响。通过纳入免疫捕获的改良试验方案提高了ELISA的特异性。
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