Mimori K, Mori M, Tanaka S, Akiyoshi T, Sugimachi K
Department of Surgery, Kyushu University, Beppu, Japan.
Cancer. 1995 Mar 15;75(6 Suppl):1446-9. doi: 10.1002/1097-0142(19950315)75:6+<1446::aid-cncr2820751509>3.0.co;2-p.
Elongation factor 1 gamma (EF1 gamma) has been isolated from a pancreatic cancer cell line, while the overexpression of EF1 gamma has also been observed in colorectal carcinomas and adenomas. There is, however, still little information on the expression of EF1 gamma in gastric carcinoma.
The authors examined the expression of EF1 gamma mRNA in 30 gastric carcinomas and their corresponding paired adjacent normal mucosae obtained during surgery while also examining 10 human digestive tract carcinoma cell lines using Northern blot hybridization.
An overexpression was observed in 22 (73%) of 30 gastric carcinomas, relative to the corresponding normal tissue. In a clinicopathologic study, no relationship was observed between the overexpression of EF1 gamma mRNA and the histologic grading or depth of tumor invasion. However, 15 (68%) of 22 overexpressed cases were positive for vascular permeation, whereas only 1 of 8 nonoverexpressed cases was positive for vascular permeation, and the difference was significant (P < 0.05). Nine (90%) of 10 with more than a 10-fold overexpression relative to the corresponding normal tissue showed severe vascular permeation in the histologic specimens. Conversely, the expression of EF1 gamma was observed not only in the pancreatic and gastric carcinoma cell lines, but also in the hepatic, ileocecal, duodenal, and colon carcinoma cell lines.
The overexpression of EF1 gamma mRNA was observed in both the gastric carcinoma tissue and in all the other cell lines examined. This probable correlation with vascular permeation in gastric carcinoma is thus considered to provide another piece of useful pathologic information.
已从胰腺癌细胞系中分离出延伸因子1γ（EF1γ），同时在结直肠癌和腺瘤中也观察到EF1γ的过表达。然而，关于EF1γ在胃癌中的表达情况仍知之甚少。
作者通过Northern印迹杂交检测了30例胃癌及其手术中获取的相应配对的癌旁正常黏膜组织中EF1γ mRNA的表达，并检测了10种人消化道癌细胞系。
相对于相应的正常组织，在30例胃癌中有22例（73%）观察到过表达。在一项临床病理研究中，未观察到EF1γ mRNA的过表达与组织学分级或肿瘤浸润深度之间存在关联。然而，在22例过表达病例中有15例（68%）血管侵犯呈阳性，而在8例未过表达病例中只有1例血管侵犯呈阳性，差异具有统计学意义（P<0.05）。相对于相应正常组织过表达超过10倍的10例病例中有9例（90%）在组织学标本中显示出严重的血管侵犯。相反，不仅在胰腺癌细胞系和胃癌细胞系中观察到了EF1γ的表达，在肝癌细胞系、回盲部癌细胞系、十二指肠癌细胞系和结肠癌细胞系中也观察到了其表达。
在胃癌组织以及所有检测的其他细胞系中均观察到了EF1γ mRNA的过表达。因此，这种与胃癌血管侵犯可能存在的相关性被认为提供了另一条有用的病理信息。
