Mimori K, Mori M, Inoue H, Ueo H, Mafune K, Akiyoshi T, Sugimachi K
Department of Surgery, Kyushu University, Beppu, Japan.
Gut. 1996 Jan;38(1):66-70. doi: 10.1136/gut.38.1.66.
Elongation factor 1 gamma (EF1 gamma) is known to be a subunit of EF1, one of the G proteins that mediate the transport of aminoacyl tRNA to 80S ribosomes during translation. As little is known regarding the expression of EF1 gamma in human oesophageal carcinoma, this study looked at its expression using a northern blot analysis. Thirty six cases of oesophageal carcinoma and 15 oesophageal carcinoma cell lines were studied. The EF1 gamma mRNA overexpression at a level of twofold or more was seen in five (14%) of 36 carcinomatous tissues compared with the normal counterparts. All five overexpressed cases showed severe lymph node metastases compared with the non-overexpressed cases, and the difference was significant (p = 0.028). The stage of the disease of these five cases was far advanced compared with the nonoverexpressed cases (p = 0.012). All 15 oesophageal carcinoma cells expressed EF1 gamma mRNA relatively lower than the gastric or pancreatic carcinoma cell lines, in which EF1 gamma was originally isolated. As the expression of EF1 gamma mRNA could be detected even in the biopsy specimens, its overexpression in tumour tissue may provide preoperative useful information for predicting the aggressiveness of tumours.
延伸因子1γ（EF1γ）是EF1的一个亚基，EF1是一种G蛋白，在翻译过程中介导氨酰tRNA转运至80S核糖体。由于对EF1γ在人食管癌中的表达了解甚少，本研究采用Northern印迹分析观察其表达情况。研究了36例食管癌及15种食管癌细胞系。与正常组织相比，36例癌组织中有5例（14%）出现EF1γ mRNA两倍或更高水平的过表达。与未过表达的病例相比，所有5例过表达病例均有严重的淋巴结转移，差异有统计学意义（p = 0.028）。这5例病例的疾病分期与未过表达病例相比进展更为严重（p = 0.012）。所有15种食管癌细胞表达的EF1γ mRNA相对低于最初分离出EF1γ的胃癌或胰腺癌细胞系。由于在活检标本中也能检测到EF1γ mRNA的表达，其在肿瘤组织中的过表达可能为预测肿瘤侵袭性提供术前有用信息。
