用于厌氧细菌生化特性分析的半微量技术
Semi-microtechnique for the biochemical characterization of anaerobic bacteria.
作者信息
Morgan J R, Liu P Y, Smith J A
出版信息
J Clin Microbiol. 1976 Oct;4(4):315-8. doi: 10.1128/jcm.4.4.315-318.1976.
Abstract
A semi-micromethod is described for characterizing anaerobic bacteria by substrate utilization. Small volumes of individual substrates were placed in the wells of plastic microtiter trays. When heavy inocula and a colorimetric indicator were used, complete results were available after 30 h of incubation. Microtiter trays containing a range of substrates can be stored at -20 degrees C. The method is accurate, economical, and convenient for use in a hospital microbiology laboratory.
摘要
描述了一种通过底物利用来鉴定厌氧细菌的半微量方法。将少量的各个底物置于塑料微量滴定板的孔中。当使用大量接种物和比色指示剂时，培养30小时后即可获得完整结果。含有一系列底物的微量滴定板可在-20℃下储存。该方法准确、经济，便于在医院微生物实验室使用。
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3
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