一种用于鉴定厌氧菌的微量方法的开发。
Development of a micromethod for identification of anaerobic bacteria.
作者信息
Wilkins T D, Walker C B
出版信息
Appl Microbiol. 1975 Nov;30(5):825-30. doi: 10.1128/am.30.5.825-830.1975.
Abstract
A microprocedure was described for determining the carbohydrate fermentation patterns of 48 anaerobic bacteria at one time in microtiter plates. The cultures were transferred into agar-filled wells of microtiter plates with a replicator inside an anaerobic glove box. Fermentation was measured both with a colorimetric indicator and with a small pH electrode. The method was approximately 97% accurate. It would be most useful for laboratories that need to identify large numbers of anaerobes at one time.
摘要
描述了一种微量程序，可一次性在微量滴定板中测定48种厌氧菌的碳水化合物发酵模式。在厌氧手套箱内，使用复制器将培养物转移到装有琼脂的微量滴定板孔中。使用比色指示剂和小型pH电极测量发酵情况。该方法的准确率约为97%。对于需要一次性鉴定大量厌氧菌的实验室来说，它将非常有用。
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