Fritzsche E, Hayatsu H, Igloi G L, Iida S, Kössel H
Nucleic Acids Res. 1987 Jul 24;15(14):5517-28. doi: 10.1093/nar/15.14.5517.
The use of permanganate as a reagent for DNA sequencing by chemical degradation has been studied with respect to its specificity for 5-methylcytosine residues. At weakly acidic pH and room temperature, 0.2 mM potassium permanganate reacts preferentially with thymine, 5-methylcytosine, and to a lesser extent with purine residues, while cytosine remains essentially intact. Permanganate oxidation is, therefore, a suitable DNA sequencing reaction for positive discrimination between 5-methylcytosine and unmethylated cytosine.
关于高锰酸钾作为化学降解法DNA测序试剂对5-甲基胞嘧啶残基的特异性，已进行了研究。在弱酸性pH和室温条件下，0.2 mM高锰酸钾优先与胸腺嘧啶、5-甲基胞嘧啶反应，与嘌呤残基的反应程度较小，而胞嘧啶基本保持完整。因此，高锰酸钾氧化是一种适用于对5-甲基胞嘧啶和未甲基化胞嘧啶进行阳性鉴别的DNA测序反应。
