Tsuchiya T, Horii I
Shiseido Research Center, Yokohama, Japan.
Psychoneuroendocrinology. 1995;20(2):221-30. doi: 10.1016/0306-4530(94)00055-f.
The effects of immobilization-induced stress on plasma testosterone levels and lipogenesis in the sebaceous gland were examined in male Syrian hamsters. To induce immobilization stress, the animals were placed in the prone position and wrapped with flexible steel wire gauze at room temperature. Plasma testosterone levels were determined by radioimmunoassay (RIA) of blood samples and sebaceous lipogenesis was determined by measurement of the incorporation of a lipid precursor, 14C-acetate, in ear skin biopsy samples. Both specimens were obtained immediately after the hamsters were decapitated. Immobilization stress for 4 consecutive days produced a marked fall in plasma testosterone levels and sebaceous lipogenesis. These reductions were reversible, the decreased plasma testosterone levels and decreased sebaceous lipogenesis recovering to the nonstressed or prestimulus levels after approximately 1 week. In 4-day stressed animals in occurrence with a decrease in plasma testosterone, sebaceous lipogenesis in ear with topical application of a small dose of testosterone, which have no effect on plasma testosterone level, was equivalent to that in nonstressed animals. These findings indicated that immobilization-induced stress lowered testosterone secretion, and consequently the testosterone levels in the skin, resulting in decreased lipogenesis in the skin. These results thus suggest that psychological or physiological stress can influence cutaneous function by inducing changes in the neuroendocrine system.
在雄性叙利亚仓鼠中研究了制动诱导应激对血浆睾酮水平和皮脂腺脂肪生成的影响。为了诱导制动应激，将动物置于俯卧位，并在室温下用柔性钢丝网包裹。通过对血样进行放射免疫测定（RIA）来测定血浆睾酮水平，并通过测量耳部皮肤活检样本中脂质前体14C-乙酸盐的掺入量来测定皮脂腺脂肪生成。这两个样本均在仓鼠断头后立即获取。连续4天的制动应激导致血浆睾酮水平和皮脂腺脂肪生成显著下降。这些降低是可逆的，血浆睾酮水平降低和皮脂腺脂肪生成减少在大约1周后恢复到非应激或刺激前水平。在血浆睾酮降低的4天应激动物中，局部应用小剂量睾酮（对血浆睾酮水平无影响）后耳部的皮脂腺脂肪生成与非应激动物相当。这些发现表明，制动诱导的应激降低了睾酮分泌，从而降低了皮肤中的睾酮水平，导致皮肤中脂肪生成减少。因此，这些结果表明，心理或生理应激可通过诱导神经内分泌系统的变化来影响皮肤功能。
