酿酒酵母第三条染色体着丝粒右侧一个10.8kb片段的完整序列。

The complete sequence of a 10.8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae.

作者信息

Biteau N, Fremaux C, Hebrard S, Menara A, Aigle M, Crouzet M

机构信息

Laboratoire de Biologie Moléculaire et de Séquençage, Université de Bordeaux II, France.

出版信息

Yeast. 1992 Jan;8(1):61-70. doi: 10.1002/yea.320080107.

DOI:10.1002/yea.320080107

PMID:1580102

Abstract

The complete nucleotide sequence of the D10H fragment (10850 bp) was determined. The D10H fragment is located on the right arm of chromosome III near the centromere and contains the SUF2 gene. Six open reading frames (ORFs) larger than 300 bp were found. One of them is the CIT2 gene encoding the cytoplasmic citrate synthase. The others are new putative genes and show no significant similarity with any known gene. In addition two tRNA genes (Asn and Pro) and a solo delta element were identified. Two ORFs were disrupted; no peculiar phenotype was observed.

摘要

测定了D10H片段（10850 bp）的完整核苷酸序列。D10H片段位于第三条染色体右臂靠近着丝粒的位置，包含SUF2基因。发现了6个大于300 bp的开放阅读框（ORF）。其中一个是编码细胞质柠檬酸合酶的CIT2基因。其他的是新的假定基因，与任何已知基因均无明显相似性。此外，还鉴定出两个tRNA基因（Asn和Pro）和一个单独的δ元件。两个ORF被破坏；未观察到特殊表型。