Robbins M E, Bywaters T, Jaenke R S, Rezvani M, Golding S J, Whitehouse E, Hopewell J W
CRC Normal Tissue Radiobiology Research Group, Research Institute, University of Oxford, UK.
Int J Radiat Biol. 1993 Oct;64(4):407-16. doi: 10.1080/09553009314551591.
Nine mature, approximately 45-week-old pigs, were fed a standard diet (SD) containing 16% protein; 10 pigs were fed an isocaloric low protein (LP) pig feed containing 4% protein 2 weeks prior to and 16 weeks after renal or sham-irradiation. The pigs then received the SD for a further 4 weeks. Both kidneys of seven pigs fed the LP diet, and six pigs fed the SD, were irradiated with a single dose of 9.8 Gy of 60Co gamma-rays. The remaining pigs received sham-irradiation. The individual kidney glomerular filtration rate (GFR), effective renal plasma flow (ERPF), and the haematocrit were serially measured before and up to 20 weeks after irradiation. The radiation-induced reduction in mean individual kidney GFR, expressed as either absolute values or as a percentage of the respective sham-irradiated controls, was significantly greater in pigs fed the SD compared with pigs fed the LP diet (p < 0.05). In contrast, the radiation-induced reduction in mean individual kidney ERPF, expressed as absolute values, observed in pigs fed the SD was not significantly different from that seen in pigs fed the LP diet. However, if these data were expressed as a percentage of the respective sham-irradiated controls, then a significantly greater reduction in ERPF was seen in pigs fed the SD compared with pigs fed the LP diet (p < 0.001). Morphological analysis of renal tissue removed 20 weeks after irradiation showed that the extent and severity of the glomerular lesions seen in pigs fed the SD were significantly reduced in pigs fed the LP diet. Thus dietary protein restriction appears to reduce some of the signs of radiation nephropathy in the pig.
9头成熟的、约45周龄的猪，喂食含16%蛋白质的标准日粮（SD）；10头猪在肾脏照射或假照射前2周及照射后16周，喂食含4%蛋白质的等热量低蛋白（LP）猪饲料。然后这些猪再接受4周的SD日粮。给7头喂食LP日粮的猪和6头喂食SD日粮的猪的双肾单次照射9.8 Gy的60Coγ射线。其余猪接受假照射。在照射前及照射后长达20周连续测量个体肾脏的肾小球滤过率（GFR）、有效肾血浆流量（ERPF）和血细胞比容。与喂食LP日粮的猪相比，喂食SD日粮的猪辐射诱导的个体肾脏平均GFR降低，以绝对值或相对于各自假照射对照的百分比表示，均显著更大（p<0.05）。相反，喂食SD日粮的猪观察到的辐射诱导的个体肾脏平均ERPF降低，以绝对值表示，与喂食LP日粮的猪没有显著差异。然而，如果将这些数据表示为相对于各自假照射对照的百分比，那么与喂食LP日粮的猪相比，喂食SD日粮的猪ERPF的降低显著更大（p<0.001）。照射后20周取出的肾脏组织的形态学分析表明，喂食LP日粮的猪肾小球病变的程度和严重程度在喂食SD日粮的猪中显著减轻。因此，饮食蛋白质限制似乎可以减轻猪辐射性肾病的一些症状。
