Lee J D, Shin K H, Cho S N, Shin J S, Lee M G, Yang W I, Park C Y, Yoo H S, Lee J T, Awh O D
Department of Diagnostic Radiology, Yonsei University, College of Medicine, Seoul, Seodaemun-gu, Korea.
Eur J Nucl Med. 1992;19(12):1011-5. doi: 10.1007/BF00180861.
Immunoscintigraphy with radiolabelled monoclonal antibodies is widely used to detect solid tumours, but only a few trials have been carried out concerning the specific in vivo localization of an inflammatory process. The purpose of this study was to investigate the detectability of tuberculous foci utilizing this method with radiolabelled bacillus Calmette-Guérin (BCG)-specific F(ab')2 in rabbits. All of the tuberculous lesions (n = 8) were clearly visualized on serial scintigraphy for up to 48 h after injection of the antibody. Immunohistochemical and Ziel-Neelson staining of the tuberculous lesions confirmed the presence of the tuberculous antigens and bacilli. It failed to demonstrate any sustained retention of the BCG-specific antibody fragment in the control group with syphilitic orchitis (n = 2). Therefore, the specific in vivo localization of tuberculosis is feasible by immunoscintigraphy.
用放射性标记的单克隆抗体进行免疫闪烁显像广泛用于检测实体瘤，但关于炎症过程的特异性体内定位仅进行了少数试验。本研究的目的是利用放射性标记的卡介苗（BCG）特异性F(ab')2在兔体内研究结核病灶的可检测性。注射抗体后长达48小时的系列闪烁显像中，所有结核病灶（n = 8）均清晰可见。结核病灶的免疫组织化学和齐-尼氏染色证实存在结核抗原和杆菌。在患有梅毒性睾丸炎的对照组（n = 2）中，未显示出BCG特异性抗体片段有任何持续留存。因此，通过免疫闪烁显像对结核病进行特异性体内定位是可行的。
