Aukrust P, Liabakk N B, Müller F, Lien E, Espevik T, Frøland S S
Section of Clinical Immunology and Infectious Diseases, Medical Department A, Rikshospitalet, Oslo, Norway.
J Infect Dis. 1994 Feb;169(2):420-4. doi: 10.1093/infdis/169.2.420.
Two EIAs (Medgenix and Quantikine) and a bioassay were used to measure tumor necrosis factor-alpha (TNF alpha) in serum samples from 73 human immunodeficiency virus type 1 (HIV-1)-seropositive patients and in samples from 2 control groups. All clinical groups of HIV-1-infected patients, regardless of concurrent illness, had significantly elevated levels of both types of soluble TNF receptors (sTNFRs) and immunoreactive TNF alpha (Medgenix EIA), with the highest concentrations among the AIDS patients. These TNF parameters were significantly correlated with reduced CD4+ lymphocyte counts. Only a few HIV-1-infected patients had detectable TNF alpha levels measured by the Quantikine EIA. TNF alpha bioactivity was significantly raised only in the AIDS group. Serially measured sTNFRs, expressed as sTNFR slopes, were significantly associated with survival in the patient group. The raised levels of immunoreactive TNF alpha and sTNFRs strongly indicate activation of the TNF alpha system during HIV-1 infection. Levels increase with disease progression and degree of immunodeficiency; thus, serially measured sTNFRs may give useful prognostic information in HIV-1 infection.
使用两种酶免疫测定法（Medgenix和Quantikine）以及一种生物测定法，对73例1型人类免疫缺陷病毒（HIV-1）血清反应阳性患者的血清样本以及2个对照组的样本进行肿瘤坏死因子-α（TNFα）检测。所有HIV-1感染患者的临床组，无论是否并发疾病，两种可溶性TNF受体（sTNFRs）和免疫反应性TNFα（Medgenix酶免疫测定法）水平均显著升高，其中艾滋病患者的浓度最高。这些TNF参数与CD4 +淋巴细胞计数减少显著相关。通过Quantikine酶免疫测定法，只有少数HIV-1感染患者可检测到TNFα水平。仅艾滋病组的TNFα生物活性显著升高。以sTNFR斜率表示的连续测量的sTNFRs与患者组的生存率显著相关。免疫反应性TNFα和sTNFRs水平升高强烈表明HIV-1感染期间TNFα系统被激活。水平随疾病进展和免疫缺陷程度增加；因此，连续测量的sTNFRs可能为HIV-1感染提供有用的预后信息。
