Leonard R B, Nowowiejski D J, Warren J J, Finn D J, Coyle M B
Department of Laboratory Medicine, Harborview Medical Center, University of Washington, Seattle 98104.
J Clin Microbiol. 1994 Jan;32(1):164-9. doi: 10.1128/jcm.32.1.164-169.1994.
During a 14-month period, a unique strain of Corynebacterium striatum that produces a diffusible brown pigment was isolated from purulent sputa of nine patients and from nonrespiratory sites of two additional patients. Seven nonpigmented clinical isolates from the same period and three reference strains of C. striatum were compared with the brown isolates. Most patients had multiple sputum cultures with no coryneforms before the brown strain emerged, suggesting that the organism was hospital acquired. DNA restriction fragment patterns and Southern hybridization with the att site probe of Corynebacterium diphtheriae indicated that the brown isolates were a single strain which was distinct from the heterogeneous nonpigmented strains. A common source for the brown C. striatum was not recognized, although all of these patients were located in two adjoining intensive care units. All of the brown isolates, three of the nonpigmented clinical isolates, and two reference strains had positive CAMP reactions with Staphylococcus aureus, which has not been reported for C. striatum prior to this study.
在14个月的时间里，从9例患者的脓性痰液以及另外2例患者的非呼吸道部位分离出一种独特的产可扩散棕色色素的纹带棒状杆菌菌株。将同期的7株无色素临床分离株和3株纹带棒状杆菌参考菌株与棕色分离株进行了比较。大多数患者在棕色菌株出现之前多次进行痰培养，均未发现棒状杆菌，提示该菌是医院获得性的。DNA限制性片段图谱以及用白喉棒状杆菌附着位点探针进行的Southern杂交表明，棕色分离株是一个单一菌株，与异质性无色素菌株不同。尽管所有这些患者都在两个相邻的重症监护病房，但未发现棕色纹带棒状杆菌的共同来源。所有棕色分离株、3株无色素临床分离株和2株参考菌株与金黄色葡萄球菌的CAMP反应均为阳性，在本研究之前纹带棒状杆菌尚未有此报道。
