Beati L, Raoult D
Unité des Rickettsies, Faculté de Médecine, Marseille, France.
Int J Syst Bacteriol. 1993 Oct;43(4):839-40. doi: 10.1099/00207713-43-4-839.
We propose the name Rickettsia massiliae sp. nov. (type strain, Mtu1 in the Collection of the World Health Organization Collaborative Center for Rickettsial Reference, Marseille, France) for a spotted fever group rickettsia determined to be distinct from previously recognized species by the serotyping method (L. Beati, J.-P. Finidori, B. Gilot, and D. Raoult, J. Clin. Microbiol. 30:1922-1930, 1992). This rickettsia has biological characteristics similar to those of the other spotted fever group rickettsiae. In addition, a sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis, a polymerase chain reaction followed by a restriction fragment length polymorphism analysis of DNA fragments, and pulsed-field electrophoresis of the genome of R. massiliae revealed unique migration patterns distinct from those of all previously recognized spotted fever group rickettsiae. These additional characteristics (Beati et al., J. Clin. Microbiol. 30:1922-1930, 1992), together with the data usually considered sufficient for description of rickettsiae, are crucial to the proposal of this new species and should be helpful in species identification.
我们提议将一种斑点热群立克次体命名为马赛立克次体新种（模式菌株为Mtu1，保藏于法国马赛的世界卫生组织立克次体参考合作中心菌种保藏库），该立克次体通过血清分型方法被确定与先前已确认的物种不同（L. 贝亚蒂、J.-P. 菲尼多里、B. 吉洛和D. 拉乌尔，《临床微生物学杂志》30:1922 - 1930，1992年）。这种立克次体具有与其他斑点热群立克次体相似的生物学特性。此外，十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳蛋白质分析、聚合酶链反应随后对DNA片段进行限制性片段长度多态性分析以及马赛立克次体基因组的脉冲场电泳显示出独特的迁移模式，与所有先前已确认的斑点热群立克次体不同。这些额外的特性（贝亚蒂等人，《临床微生物学杂志》30:1922 - 1930，1992年），连同通常被认为足以描述立克次体的资料，对于提出这个新物种至关重要，并且应该有助于物种鉴定。
