氯霉素：一种酶学微量测定法。
Chloramphenicol: an enzymological microassay.
作者信息
Lietman P S, White T J, Shaw W V
出版信息
Antimicrob Agents Chemother. 1976 Aug;10(2):347-53. doi: 10.1128/AAC.10.2.347.
Abstract
A new assay for chloramphenicol in biological fluids has been developed that offers sensitivity, specificity, precision, accuracy, economy, and ease of performance. The assay is based on the enzymological acetylation of chloramphenicol catalyzed by an R factor-mediated enzyme. [(14)C]acetyl coenzyme A serves as the donor of the labeled acetyl group, and the product, [(14)C]acetoxychloramphenicol, is separated from the labeled precursor by utilizing its preferential extraction into benzene. The product is then quantified by liquid scintillation counting. This assay measures chloramphenicol concentrations in both plasma and other biological specimens and in the presence of other antibiotics, hemolysis, or jaundice. Its rapidity and ease of performance are useful for clinical laboratories, and its sensitivity allows determinations on 10 mul of plasma.
摘要
已开发出一种用于生物体液中氯霉素的新检测方法，该方法具有灵敏度、特异性、精密度、准确性、经济性且操作简便。该检测方法基于R因子介导的酶催化氯霉素的酶促乙酰化反应。[(14)C]乙酰辅酶A作为标记乙酰基团的供体，产物[(14)C]乙酰氧基氯霉素通过优先萃取到苯中与标记前体分离。然后通过液体闪烁计数对产物进行定量。该检测方法可测量血浆和其他生物标本中的氯霉素浓度，且不受其他抗生素、溶血或黄疸的影响。其快速性和操作简便性对临床实验室很有用，其灵敏度允许对10微升血浆进行测定。
相似文献
1
Chloramphenicol: an enzymological microassay.氯霉素：一种酶学微量测定法。
Antimicrob Agents Chemother. 1976 Aug;10(2):347-53. doi: 10.1128/AAC.10.2.347.
2
Simplified radioenzymatic assay for chloramphenicol.氯霉素简易放射酶测定法
Antimicrob Agents Chemother. 1978 Jan;13(1):25-9. doi: 10.1128/AAC.13.1.25.
3
Monitoring bacterial resistance to chloramphenicol and other antibiotics by liquid chromatography electrospray ionization tandem mass spectrometry using selected reaction monitoring.采用液质联用选择反应监测监测氯霉素和其他抗生素的细菌耐药性。
J Mass Spectrom. 2013 Jun;48(6):732-9. doi: 10.1002/jms.3220.
4
Temperature-sensitive chloramphenicol acetyltransferase from Escherichia coli carrying mutant R factors.携带突变R因子的大肠杆菌温度敏感型氯霉素乙酰转移酶
J Bacteriol. 1968 Jun;95(6):2124-30. doi: 10.1128/jb.95.6.2124-2130.1968.
5
Rapid bioassay for chloramphenicol in the presence of other antibiotics.在其他抗生素存在的情况下对氯霉素进行快速生物测定。
Am J Clin Pathol. 1981 Oct;76(4):472-5. doi: 10.1093/ajcp/76.4.472.
6
Chloramphenicol-sensitive Escherichia coli strain expressing the chloramphenicol acetyltransferase (cat) gene.表达氯霉素乙酰转移酶（cat）基因的氯霉素敏感型大肠杆菌菌株。
Antimicrob Agents Chemother. 2001 Dec;45(12):3610-2. doi: 10.1128/AAC.45.12.3610-3612.2001.
7
Chloramphenicol acetyltransferase assay.氯霉素乙酰转移酶测定
Cold Spring Harb Protoc. 2010 May;2010(5):pdb.prot5422. doi: 10.1101/pdb.prot5422.
8
Inactivation of the acrA gene is partially responsible for chloramphenicol sensitivity of Escherichia coli CM2555 strain expressing the chloramphenicol acetyltransferase gene.acrA基因的失活是表达氯霉素乙酰转移酶基因的大肠杆菌CM2555菌株对氯霉素敏感的部分原因。
Microb Drug Resist. 2002 Fall;8(3):179-85. doi: 10.1089/107662902760326887.
9
A simple quantitative assay for chloramphenicol acetyltransferase by direct extraction of the labeled product into scintillation cocktail.
Anal Biochem. 1992 Jan;200(1):180-6. doi: 10.1016/0003-2697(92)90296-j.
10
The acrAB locus is involved in modulating intracellular acetyl coenzyme A levels in a strain of Escherichia coli CM2555 expressing the chloramphenicol acetyltransferase (cat) gene.acrAB基因座参与调节表达氯霉素乙酰转移酶（cat）基因的大肠杆菌CM2555菌株中的细胞内乙酰辅酶A水平。
Arch Microbiol. 2003 Nov;180(5):362-6. doi: 10.1007/s00203-003-0592-x. Epub 2003 Sep 3.
引用本文的文献
1
Set-base dynamical parameter estimation and model invalidation for biochemical reaction networks.生化反应网络的基于集合的动力学参数估计与模型验证
BMC Syst Biol. 2010 May 25;4:69. doi: 10.1186/1752-0509-4-69.
2
Comparison of cotrimoxazole, ampicillin, and chloramphenicol in treatment of experimental Haemophilus influenzae type B meningitis.复方新诺明、氨苄青霉素和氯霉素治疗实验性B型流感嗜血杆菌脑膜炎的比较。
Antimicrob Agents Chemother. 1980 Jan;17(1):43-8. doi: 10.1128/AAC.17.1.43.
3
Bactericidal versus bacteriostatic antibiotic therapy of experimental pneumococcal meningitis in rabbits.兔实验性肺炎球菌性脑膜炎的杀菌与抑菌抗生素治疗
J Clin Invest. 1983 Mar;71(3):411-9. doi: 10.1172/jci110785.
4
Antimicrobial therapy in newborn infants.新生儿抗菌治疗
Indian J Pediatr. 1983 Mar-Apr;50(403):183-93. doi: 10.1007/BF02821441.
5
Clinical pharmacokinetics of chloramphenicol and chloramphenicol succinate.氯霉素及琥珀氯霉素的临床药代动力学
Clin Pharmacokinet. 1984 May-Jun;9(3):222-38. doi: 10.2165/00003088-198409030-00004.
6
Detection and quantitation of chloramphenicol by competitive enzyme-linked immunoassay.采用竞争性酶联免疫分析法检测和定量氯霉素。
Antimicrob Agents Chemother. 1984 Feb;25(2):205-11. doi: 10.1128/AAC.25.2.205.
7
Comparison of methods available for assay of chloramphenicol in clinical specimens.临床样本中氯霉素检测可用方法的比较。
J Clin Pathol. 1980 Jun;33(6):575-80. doi: 10.1136/jcp.33.6.575.
8
Simple method for elimination of aminoglycosides from serum to permit bioassay of other antimicrobial agents.从血清中去除氨基糖苷类以允许对其他抗菌剂进行生物测定的简单方法。
Antimicrob Agents Chemother. 1977 Aug;12(2):286-7. doi: 10.1128/AAC.12.2.286.
9
High-performance liquid chromatographic assay of chloramphenicol in serum.血清中氯霉素的高效液相色谱测定法
Antimicrob Agents Chemother. 1978 Sep;14(3):439-43. doi: 10.1128/AAC.14.3.439.
10
Simplified radioenzymatic assay for chloramphenicol.氯霉素简易放射酶测定法
Antimicrob Agents Chemother. 1978 Jan;13(1):25-9. doi: 10.1128/AAC.13.1.25.
本文引用的文献
1
A colorimetric method for the determination of chloromycetin in serum or plasma.一种测定血清或血浆中氯霉素的比色法。
J Lab Clin Med. 1950 Jan;35(1):129-35.
2
[Absorption and excretion of drugs. V. Determination of chloramphenicol in urine and its metabolic rate].
Yakugaku Zasshi. 1962 Mar;82:345-8.
3
CHLORAMPHENICOL IN BLOOD: SIMPLE CHEMICAL ESTIMATIONS IN PATIENTS RECEIVING MULTIPLE ANTIBIOTICS.血液中的氯霉素：接受多种抗生素治疗患者的简单化学测定
Science. 1964 Apr 17;144(3616):296-7. doi: 10.1126/science.144.3616.296.
4
Determination of chloramphenicol by gas-liquid chromatography.气液色谱法测定氯霉素
Chem Pharm Bull (Tokyo). 1967 Jan;15(1):123-7. doi: 10.1248/cpb.15.123.
5
Determination of serum chloramphenicol utilizing gas--liquid chromatography and electron capture spectrometry.
Anal Chem. 1966 Apr;38(4):582-5. doi: 10.1021/ac60236a014.
6
The enzymatic acetylation of chloramphenicol by extracts of R factor-resistant Escherichia coli.R因子抗性大肠杆菌提取物对氯霉素的酶促乙酰化作用。
J Biol Chem. 1967 Feb 25;242(4):687-93.
7
The enzymatic acetylation of chloramphenicol by the multiple drug-resistant Escherichia coli carrying R factor.携带R因子的多重耐药性大肠杆菌对氯霉素的酶促乙酰化作用。
J Biol Chem. 1967 Oct 25;242(20):4722-30.
8
GLC analysis of chloramphenicol: a collaborative study.氯霉素的气相色谱分析：一项合作研究。
J Pharm Sci. 1974 Mar;63(3):435-7. doi: 10.1002/jps.2600630327.
9
The release of enzymes by osmotic shock from Escherichia coli in exponential phase.处于对数期的大肠杆菌通过渗透休克释放酶。
J Biol Chem. 1966 Jul 10;241(13):3055-62.
10
Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.来自耐氯霉素细菌的氯霉素乙酰转移酶。
Methods Enzymol. 1975;43:737-55. doi: 10.1016/0076-6879(75)43141-x.
Zotero 插件