Tonokatsu Y, Hayashi T, Fukuda Y, Tamura T, Shimoyama T
Fourth Department of Internal Medicine, Hyogo College of Medicine, Japan.
J Gastroenterol. 1994 Apr;29(2):120-4. doi: 10.1007/BF02358671.
Helicobacter pylori (H. pylori) is a gram-negative bacillus thought to be involved in such diseases of the upper gastrointestinal tract as gastritis, peptic ulcers, and gastric cancer. Urease is regarded as the factor responsible for the pathogenic nature of this bacterium. Therefore, in our examination of the genetic polymorphism of H. pylori, by means of Southern blotting, we used the urease gene as a probe. The Southern blot patterns of H. pylori isolated from different patients differed greatly, the inter-individual variation being so marked that it allowed approximate distinction between individual patients. The Southern blot patterns of individual strains of H. pylori did not change, even when they were stored and passed from generation to generation in our laboratory. These results suggest that DNA fingerprints with a urease gene probe will be useful in epidemiologically tracing H. pylori infection. Almost all strains of H. pylori isolated from different sites in the stomach of a patient on different occasions showed the same pattern, allowing us to confirm that only one strain of H. pylori was responsible for H. pylori infection in individual patients.
幽门螺杆菌（H. pylori）是一种革兰氏阴性杆菌，被认为与上消化道疾病如胃炎、消化性溃疡和胃癌有关。脲酶被视为该细菌致病特性的相关因素。因此，在我们对幽门螺杆菌基因多态性的检测中，通过Southern印迹法，我们使用脲酶基因作为探针。从不同患者分离出的幽门螺杆菌的Southern印迹图谱差异很大，个体间差异非常明显，以至于可以对个体患者进行大致区分。即使在我们实验室中储存并传代，幽门螺杆菌各个菌株的Southern印迹图谱也没有改变。这些结果表明，用脲酶基因探针进行DNA指纹分析将有助于对幽门螺杆菌感染进行流行病学追踪。从同一患者胃内不同部位在不同时间分离出的几乎所有幽门螺杆菌菌株都显示出相同的图谱，这使我们能够确认在个体患者中只有一种幽门螺杆菌菌株导致幽门螺杆菌感染。
