Inouye H, Yamamoto I, Tanida N, Mikami J, Tamura K, Ohno T, Kano M, Shimoyama T
Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Japan.
Gastroenterol Jpn. 1989 Oct;24(5):494-504. doi: 10.1007/BF02773875.
The presence of Campylobacter pylori was investigated in biopsy specimens obtained during gastrofiberscopy from 103 consecutive patients prospectively. Patients included 25 with gastric ulcer, 4 with duodenal ulcer, 5 with coexisting gastroduodenal ulcer, 31 with gastroduodenal ulcer with gastritis, 27 with gastritis, 3 with gastric polyps and 8 with gastric cancer. Results were compared with 20 healthy control subjects who were endoscopically normal. Two specimens each were taken from 3 sites in the stomach. One part was used for a histological study to examine the presence of the organisms. The other part was cultured using Skirrow's agar microaerophilically. Conventional microflora and C. pylori were examined in gastric contents of some cases. Bacteriological features of isolated strains of C. pylori were identical to the NCTC strain. C. pylori was the most dominant organism in gastric contents at any pH level. Detection rates of C. pylori by bacteriological culture were 96% in gastric ulcer, 100% in duodenal ulcer, 80% in coexisting gastroduodenal ulcer, 84% in gastroduodenal ulcer with gastritis, 70% in gastritis, 100% in gastric polyps and 100% in gastric cancer, and the percentages recognized by histological studies were 81, 100, 100, 84, 71, 67, and 57%, respectively. The values in healthy controls were 55% by histological and bacteriological methods (P less than 0.001 compared with overall ulcer patients). These results supported the close association between C. pylori and gastroduodenal diseases.
前瞻性地对103例连续接受纤维胃镜检查的患者活检标本进行幽门螺杆菌检测。患者包括25例胃溃疡患者、4例十二指肠溃疡患者、5例胃十二指肠溃疡并存患者、31例胃十二指肠溃疡合并胃炎患者、27例胃炎患者、3例胃息肉患者和8例胃癌患者。结果与20例内镜检查正常的健康对照者进行比较。从胃的3个部位各取两份标本。一份用于组织学研究以检查细菌的存在情况。另一份在微需氧条件下用斯氏琼脂培养基培养。对部分病例的胃内容物进行常规微生物群和幽门螺杆菌检查。分离出的幽门螺杆菌菌株的细菌学特征与英国国家标准菌种收藏中心（NCTC）菌株相同。在任何pH水平下，幽门螺杆菌都是胃内容物中最主要的细菌。通过细菌培养检测幽门螺杆菌的阳性率在胃溃疡患者中为96%，十二指肠溃疡患者中为100%，胃十二指肠溃疡并存患者中为80%，胃十二指肠溃疡合并胃炎患者中为84%，胃炎患者中为70%，胃息肉患者中为100%，胃癌患者中为100%，而通过组织学研究识别的百分比分别为81%、100%、100%、84%、71%、67%和57%。健康对照者通过组织学和细菌学方法检测的阳性率为55%（与所有溃疡患者相比，P<0.001）。这些结果支持了幽门螺杆菌与胃十二指肠疾病之间的密切关联。
