Barrenechea M A, López J, Martínez A
Department of Cytology and Histology, University of Navarra, Pamplona, Spain.
Tissue Cell. 1994 Jun;26(3):309-21. doi: 10.1016/0040-8166(94)90017-5.
The endocrine cells of the rainbow trout (Oncorhynchus mykiss) stomach have been investigated using the immunocytochemical techniques of peroxidase-anti-peroxidase and avidin-biotin-peroxidase complexes on paraffin sections. 33 antisera were tested and eight immunoreactivities were detected: somatostatin-, glucagon- bombesin-, substance P-, serotonin-, met-enkephalin-, CCK/gastrin-, and chromogranin-like containing cells. All of them were present throughout the gastric mucosa except CCK/gastrin-like containing cells that were restricted to the pyloric epithelium. Somatostatin 25 and chromogranin immunoreactive cells are described for the first time in fish stomach. Serotonin immunoreactive cells were also positive for the Grimelius technique and some of them were immunoreactive to anti substance P or anti CCK/gastrin. Immunoreactivities for gastrin 17, gastrin 34 and CCK appeared in the same cells and the absorption controls showed that a molecule containing the carboxi-terminal pentapeptide of this family was present in trout stomach.
利用过氧化物酶 - 抗过氧化物酶和抗生物素蛋白 - 生物素 - 过氧化物酶复合物免疫细胞化学技术，对虹鳟（Oncorhynchus mykiss）胃的内分泌细胞进行了石蜡切片研究。检测了33种抗血清，发现了8种免疫反应性：生长抑素、胰高血糖素、蛙皮素、P物质、5-羟色胺、甲硫氨酸脑啡肽、胆囊收缩素/胃泌素和嗜铬粒蛋白样细胞。除了局限于幽门上皮的胆囊收缩素/胃泌素样细胞外，所有这些细胞均存在于整个胃黏膜中。生长抑素25和嗜铬粒蛋白免疫反应性细胞在鱼类胃中首次被描述。5-羟色胺免疫反应性细胞对Grimelius技术也呈阳性，其中一些对P物质抗体或胆囊收缩素/胃泌素抗体呈免疫反应性。胃泌素17、胃泌素34和胆囊收缩素的免疫反应性出现在相同的细胞中，吸收对照显示虹鳟胃中存在含有该家族羧基末端五肽的分子。
