Yogo Y, Guo J, Iida T, Satoh K, Taguchi F, Takahashi H, Hall W W, Nagashima K
Department of Viral Infection, University of Tokyo, Japan.
Virus Genes. 1994 Mar;8(2):99-105. doi: 10.1007/BF01703608.
We established 99 JC virus (JCV) DNA clones directly from the brain of a single patient with progressive multifocal leukoencephalopathy (PML). Based upon restriction patterns, the cloned viral DNAs were classified into two major groups (NY-1A and -1B) containing 53 and 35 clones, respectively, and several minor groups containing one or a few clones. The regulatory sequences of representative clones were compared with the archetypal regulatory sequence, which has been detected in JCV DNAs cloned from the urine of healthy and nonimmunosuppressed individuals. The regulatory sequence of NY-1B had the two structural features common to most PML-type regulatory regions, duplication and deletion of specific segments in the archetypal sequence, while that of NY-1A contained a small deletion and an insertion of a 29-bp sequence originating from the early region of the JCV genome. A regulatory region similar to that of NY-1A has never been detected in JCV isolates obtained thus far.
我们直接从一名进行性多灶性白质脑病（PML）患者的大脑中建立了99个JC病毒（JCV）DNA克隆。根据限制性酶切图谱，克隆的病毒DNA被分为两个主要组（NY - 1A和 - 1B），分别包含53个和35个克隆，以及几个包含一个或几个克隆的次要组。将代表性克隆的调控序列与原型调控序列进行比较，该原型调控序列已在从健康和非免疫抑制个体尿液中克隆的JCV DNA中检测到。NY - 1B的调控序列具有大多数PML型调控区域共有的两个结构特征，即原型序列中特定片段的重复和缺失，而NY - 1A的调控序列包含一个小的缺失和一个源自JCV基因组早期区域的29个碱基对序列的插入。迄今为止，在JCV分离株中从未检测到与NY - 1A类似的调控区域。
