Woodruff-Pak D S, Li Y T, Kazmi A, Kem W R
Department of Psychology, Temple University, Philadelphia, Pennsylvania 19122.
Behav Neurosci. 1994 Jun;108(3):486-93. doi: 10.1037//0735-7044.108.3.486.
A nicotinic cholinergic antagonist, mecamylamine (MEC), was administered to rabbits tested on eyeblink classical conditioning (EBCC) in the 750-ms delay paradigm for 10 90-trial sessions. Nicotinic receptors were measured in 3 brain regions in 5 treatment groups: paired conditioned stimulus-unconditioned stimulus (CS-US) presentations with (a) vehicle, young; (b) vehicle, older; (c) 0.5 mg/kg MEC, young; unpaired CS-US with (d) 0.5 mg/kg MEC, young; and (e) vehicle, young. Daily MEC injections disrupted acquisition in young rabbits (769 trials to learning criterion vs. 323 trials for vehicle-treated young rabbits). MEC-treated young rabbits learned similarly to older rabbits. Brain nicotinic receptors were not affected by 10 daily MEC injections. To our knowledge, this experimental protocol, using a low MEC dose to selectively inhibit nicotinic cholinergic receptors, is the first to demonstrate a role for nicotinic cholinergic receptors in EBCC.
一种烟碱型胆碱能拮抗剂美加明（MEC），被用于对在750毫秒延迟范式下进行眨眼经典条件反射（EBCC）测试的兔子进行给药，共进行10次，每次90个试验环节。在5个治疗组的3个脑区中测量烟碱型受体：配对条件刺激-非条件刺激（CS-US）呈现，分别为（a）溶剂对照组，年轻兔子；（b）溶剂对照组，年长兔子；（c）0.5毫克/千克美加明，年轻兔子；非配对CS-US，分别为（d）0.5毫克/千克美加明，年轻兔子；以及（e）溶剂对照组，年轻兔子。每日注射美加明会干扰年轻兔子的习得过程（达到学习标准需要769次试验，而溶剂对照组的年轻兔子只需323次试验）。接受美加明治疗的年轻兔子的学习情况与年长兔子相似。每日10次注射美加明并未影响脑烟碱型受体。据我们所知，这种使用低剂量美加明选择性抑制烟碱型胆碱能受体的实验方案，首次证明了烟碱型胆碱能受体在EBCC中的作用。
