化学发光与免疫测定

Chemiluminescence and immunoassays.

作者信息

Rongen H A, Hoetelmans R M, Bult A, van Bennekom W P

机构信息

Department of Pharmaceutical Analysis, Faculty of Pharmacy, Utrecht University, The Netherlands.

出版信息

J Pharm Biomed Anal. 1994 Apr;12(4):433-62. doi: 10.1016/0731-7085(94)80027-8.

DOI:10.1016/0731-7085(94)80027-8

PMID:7918762

Abstract

The principles of chemiluminescence and the application of chemiluminescent labels and substrates in immunoassays are reviewed. Various immunoassay formats and all known classes of chemiluminescent molecules, including 1,2-dioxetanes, luminol and derivatives, acridinium esters, oxalate esters and firefly luciferins are described as well as the many sensitizers and fluorescent enhancers. Recent promising developments are discussed.

摘要

本文综述了化学发光原理以及化学发光标记物和底物在免疫分析中的应用。描述了各种免疫分析形式以及所有已知类别的化学发光分子，包括1,2 - 二氧杂环丁烷、鲁米诺及其衍生物、吖啶酯、草酸酯和萤火虫荧光素，以及许多敏化剂和荧光增强剂。还讨论了近期有前景的进展。