高氮杂甾醇介导的酵母甾醇生物合成抑制作用。
Homoazasterol-mediated inhibition of yeast sterol biosynthesis.
作者信息
Bailey R B, Hays P R, Parks L W
出版信息
J Bacteriol. 1976 Dec;128(3):730-4. doi: 10.1128/jb.128.3.730-734.1976.
Abstract
A naturally occurring azasterol has been shown to inhibit sterol transmethylation in both in vitro and in vivo in the yeast Saccharomyces cerevisiae. The inhibition was competitive, with a calculated dissociation constant of 43 muM. The compound prevented the accumulation of ergosterol in aerobically adapting cells. Cultures forced to gain energy by respiration were found to be much more sensitive to growth inhibition by the azasterol than those cells fermenting glucose. The growth inhibition is reversible at low concentrations of the azasterol.
摘要
一种天然存在的氮杂甾醇已被证明在体外和体内均能抑制酿酒酵母中的甾醇转甲基化。这种抑制是竞争性的，计算得出的解离常数为43μM。该化合物可阻止需氧适应细胞中麦角甾醇的积累。与发酵葡萄糖的细胞相比，发现通过呼吸获取能量的培养物对氮杂甾醇的生长抑制更为敏感。在低浓度的氮杂甾醇下，生长抑制是可逆的。
相似文献
1
Homoazasterol-mediated inhibition of yeast sterol biosynthesis.高氮杂甾醇介导的酵母甾醇生物合成抑制作用。
J Bacteriol. 1976 Dec;128(3):730-4. doi: 10.1128/jb.128.3.730-734.1976.
2
Physiological effects of an antimycotic azasterol on cultures of Saccharomyces cerevisiae.一种抗真菌氮杂甾醇对酿酒酵母培养物的生理效应。
Antimicrob Agents Chemother. 1977 Aug;12(2):185-91. doi: 10.1128/AAC.12.2.185.
3
Accumulation of ergosta-8,14-dien-3beta-ol by Saccharomyces cerevisiae cultured with an azasterol antimycotic agent.酿酒酵母在与氮杂甾醇抗真菌剂共培养时麦角甾-8,14-二烯-3β-醇的积累
Lipids. 1977 Aug;12(8):666-8. doi: 10.1007/BF02533762.
4
5
Sterols in yeast subcellular fractions.酵母亚细胞组分中的甾醇
Lipids. 1978 Oct;13(10):730-5. doi: 10.1007/BF02533753.
6
Azasterol inhibitors in yeast. Inhibition of the 24-methylene sterol delta24(28)-reductase and delta24-sterol methyltransferase of Saccharomyces cerevisiae by 23-azacholesterol.酵母中的氮杂甾醇抑制剂。23-氮杂胆固醇对酿酒酵母24-亚甲基甾醇δ24(28)-还原酶和δ24-甾醇甲基转移酶的抑制作用。
Biochim Biophys Acta. 1978 Jun 23;529(3):429-37. doi: 10.1016/0005-2760(78)90087-5.
7
Mode of action of the azasteroid antibiotic 15-aza-24 methylene-d-homocholesta-8,14-dien-3 beta-ol in Ustilago maydis.氮杂甾体抗生素15-氮杂-24-亚甲基-d-高胆甾-8,14-二烯-3β-醇在玉米黑粉菌中的作用方式
Antimicrob Agents Chemother. 1979 Jul;16(1):81-97. doi: 10.1128/AAC.16.1.81.
8
Relationship between antifungal activity and inhibition of sterol biosynthesis in miconazole, clotrimazole, and 15-azasterol.咪康唑、克霉唑和15-氮杂甾醇的抗真菌活性与甾醇生物合成抑制之间的关系。
Antimicrob Agents Chemother. 1983 Apr;23(4):515-21. doi: 10.1128/AAC.23.4.515.
9
Structure-function activity of azasterols and nitrogen-containing steroids.氮杂甾醇和含氮甾体的结构-功能活性
Lipids. 1976 Oct;11(10):755-62. doi: 10.1007/BF02533051.
10
Effects of an azasterol inhibitor of sterol 24-transmethylation on sterol biosynthesis and growth of Leishmania donovani promastigotes.一种甾醇24-转甲基化氮杂甾醇抑制剂对杜氏利什曼原虫前鞭毛体甾醇生物合成及生长的影响
Biochem J. 1995 May 15;308 ( Pt 1)(Pt 1):31-8. doi: 10.1042/bj3080031.
引用本文的文献
1
Biochemical and physiological effects of sterol alterations in yeast--a review.酵母中固醇变化的生化和生理效应——综述
Lipids. 1995 Mar;30(3):227-30. doi: 10.1007/BF02537825.
2
Growth and antifungal homoazasterol production in Geotrichum flavo-brunneum.黄褐地霉的生长及抗真菌高氮杂甾醇的产生
Antimicrob Agents Chemother. 1980 Nov;18(5):822-8. doi: 10.1128/AAC.18.5.822.
3
Physiological response of Saccharomyces cerevisiae to 15-azasterol-mediated growth inhibition.酿酒酵母对15-氮杂甾醇介导的生长抑制的生理反应。
Antimicrob Agents Chemother. 1981 Aug;20(2):184-9. doi: 10.1128/AAC.20.2.184.
4
Corresponding changes in kynurenine hydroxylase activity, membrane fluidity, and sterol composition in Saccharomyces cerevisiae mitochondria.酿酒酵母线粒体中犬尿氨酸羟化酶活性、膜流动性和甾醇组成的相应变化。
J Bacteriol. 1981 Mar;145(3):1325-33. doi: 10.1128/jb.145.3.1325-1333.1981.
5
Inhibition of sterol transmethylation by S-adenosylhomocysteine analogs.S-腺苷同型半胱氨酸类似物对甾醇转甲基作用的抑制
J Bacteriol. 1981 Jan;145(1):106-12. doi: 10.1128/jb.145.1.106-112.1981.
6
Self-induced nystatin resistance in Dictyostelium discoideum.盘基网柄菌中自诱导的制霉菌素抗性
Antimicrob Agents Chemother. 1985 Jun;27(6):974-6. doi: 10.1128/AAC.27.6.974.
7
Temporal expression of transcription and relative copy number of plasmid pSFB-1 in Scytalidium flavo-brunneum.黄褐帚霉中质粒pSFB - 1的转录时间表达及相对拷贝数
J Bacteriol. 1989 Sep;171(9):4876-80. doi: 10.1128/jb.171.9.4876-4880.1989.
8
Systemically administered antifungal agents. A review of their clinical pharmacology and therapeutic applications.全身用抗真菌药。其临床药理学与治疗应用综述。
Drugs. 1992 Jul;44(1):9-35. doi: 10.2165/00003495-199244010-00002.
9
Mode of action of the azasteroid antibiotic 15-aza-24 methylene-d-homocholesta-8,14-dien-3 beta-ol in Ustilago maydis.氮杂甾体抗生素15-氮杂-24-亚甲基-d-高胆甾-8,14-二烯-3β-醇在玉米黑粉菌中的作用方式
Antimicrob Agents Chemother. 1979 Jul;16(1):81-97. doi: 10.1128/AAC.16.1.81.
10
Sterols in yeast subcellular fractions.酵母亚细胞组分中的甾醇
Lipids. 1978 Oct;13(10):730-5. doi: 10.1007/BF02533753.
本文引用的文献
1
Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms.酵母及其他微生物中呼吸缺陷（RD）突变研究的进展。
Bacteriol Rev. 1961 Dec;25(4):404-26. doi: 10.1128/br.25.4.404-426.1961.
2
TRANSMETHYLATION PRODUCTS AS INTERMEDIATES IN ERGOSTEROL BIOSYNTHESIS IN YEAST.作为酵母中麦角固醇生物合成中间体的转甲基化产物
Biochim Biophys Acta. 1965 Apr 5;98:394-401. doi: 10.1016/0005-2760(65)90132-3.
3
Effect of temperature on sterol metabolism in yeast.温度对酵母中甾醇代谢的影响。
J Cell Comp Physiol. 1962 Apr;59:107-10. doi: 10.1002/jcp.1030590203.
4
Tetrazolium overlay technique for population studies of respiration deficiency in yeast.用于酵母呼吸缺陷群体研究的四氮唑覆盖技术
Science. 1957 May 10;125(3254):928-9. doi: 10.1126/science.125.3254.928.
5
Net synthesis of sterols in resting cells of Saccharomyces cerevisiae.酿酒酵母静止细胞中甾醇的净合成
Biochim Biophys Acta. 1954 Apr;13(4):591. doi: 10.1016/0006-3002(54)90382-0.
6
7
Differential effect of respiratory inhibitors on ergosterol synthesis by Saccharomyces cerevisiae during adaptation to oxygen.呼吸抑制剂对酿酒酵母在适应氧气过程中麦角固醇合成的不同影响。
J Bacteriol. 1969 Oct;100(1):370-6. doi: 10.1128/jb.100.1.370-376.1969.
8
Effect of azasterols on sterol metabolism in the laying hen.氮杂甾醇对蛋鸡甾醇代谢的影响。
Poult Sci. 1972 Mar;51(2):449-57. doi: 10.3382/ps.0510449.
9
Transmethylation of sterols in aerobically adapting Saccharomyces cerevisiae.需氧适应型酿酒酵母中固醇的甲基化作用
J Bacteriol. 1972 Jan;109(1):236-42. doi: 10.1128/jb.109.1.236-242.1972.
10
Structure of antibiotic A 25822 B, a novel nitrogen-containing C28-sterol with antifungal properties.抗生素A 25822 B的结构，一种具有抗真菌特性的新型含氮C28甾醇。
J Antibiot (Tokyo). 1974 Dec;27(12):992-3. doi: 10.7164/antibiotics.27.992.
Zotero 插件