Kondo Y, Takano F
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Biol Pharm Bull. 1994 May;17(5):759-61. doi: 10.1248/bpb.17.759.
The enhancement of nitric oxide (NO) production in glycyrrhizin (GL)-induced macrophages (M phi) in response to lipopolysaccharide (LPS) was investigated. No production in GL-induced macrophage culture supernatants was stimulated in response to LPS (10 micrograms/ml) for 24- or 48- h cultures, and these levels were compared three times with the levels in saline-induced peritoneal exudate cell cultures. Furthermore, M phi induced with proteose peptone (PP) containing GL could generate greater NO production than M phi induced with PP alone. However, no stimulation of NO production was observed by addition of GL in the cultures of M phi induced with thioglycollate or Bacillus Calmette Guerin. Moreover, GL-induced M phi showed cytostasis against such tumor target cells as L 1210 and P 388 lymphoma cell lines. These observations indicate that GL can activate the M phi in vivo system and stimulate NO production in response to LPS.
研究了甘草甜素（GL）诱导的巨噬细胞（M phi）中一氧化氮（NO）生成对脂多糖（LPS）的增强作用。在GL诱导的巨噬细胞培养上清液中，LPS（10微克/毫升）刺激24小时或48小时培养均未刺激NO生成，并且这些水平与生理盐水诱导的腹腔渗出细胞培养物中的水平进行了三次比较。此外，用含GL的蛋白胨（PP）诱导的M phi比单独用PP诱导的M phi能产生更多的NO。然而，在巯基乙酸盐或卡介苗诱导的M phi培养物中添加GL未观察到对NO生成的刺激。此外，GL诱导的M phi对L 1210和P 388淋巴瘤细胞系等肿瘤靶细胞表现出细胞停滞作用。这些观察结果表明，GL可以激活体内的M phi系统，并刺激其对LPS产生NO。
