Klein M, Eckert-Ossenkopp U, Schmiedeberg I, Brandt P, Unseld M, Brennicke A, Schuster W
Institut für Genbiologische Forschung GmbH, Berlin, Germany.
Plant J. 1994 Sep;6(3):447-55. doi: 10.1046/j.1365-313x.1994.06030447.x.
As part of the worldwide efforts at molecular analysis of Arabidopsis thaliana as a model plant the complete structure of the mitochondrial genome has been determined. The mitochondrial DNA molecules were mapped by restriction fragment analysis of more than 300 cosmid clones and purified mitochondrial DNA. The entire genome of 372 kb is contained in three different configurations of circular molecules and is split into two additional subgenomic molecules of 234 kb and 138 kb, respectively. These arrangements result from recombinations of the two sets of repeats present in combinations of inverted and/or direct orientation. Alignment of YAC clones confirms the in vivo presence of continuous DNA molecules of more than 300 kb in A. thaliana mitochondria. The presence of this comparatively large mitochondrial genome in a plant with one of the smallest nuclear genomes shows that different size constraints act upon the different genomes in plant cells.
作为全球以拟南芥为模式植物进行分子分析工作的一部分，线粒体基因组的完整结构已被确定。通过对300多个黏粒克隆和纯化的线粒体DNA进行限制性片段分析，绘制了线粒体DNA分子图谱。372 kb的整个基因组包含在三种不同构型的环状分子中，并分别被分割成另外两个亚基因组分子，大小分别为234 kb和138 kb。这些排列是由两组以反向和/或正向组合存在的重复序列重组产生的。酵母人工染色体（YAC）克隆的比对证实了拟南芥线粒体中存在长度超过300 kb的连续DNA分子。在具有最小核基因组之一的植物中存在这种相对较大的线粒体基因组，表明不同的大小限制作用于植物细胞中的不同基因组。
