系统性红斑狼疮及其他自身免疫性疾病中针对90 kDa热休克蛋白自身抗体的检测

Detection of autoantibodies to the 90 kDa heat shock protein in systemic lupus erythematosus and other autoimmune diseases.

作者信息

Conroy S E, Faulds G B, Williams W, Latchman D S, Isenberg D A

机构信息

Medical Molecular Biology Unit, Department of Molecular Pathology, London.

出版信息

Br J Rheumatol. 1994 Oct;33(10):923-6. doi: 10.1093/rheumatology/33.10.923.

DOI:10.1093/rheumatology/33.10.923

PMID:7921751

Abstract

Expression of the highly conserved 90 kDa heat shock protein (Hsp90) is elevated in the peripheral blood mononuclear cells of approximately 25% of patients with SLE. Conflicting data have been published about the frequency of antibodies to Hsp90 with the previous methodology using a complex Western blot system. We now describe an ELISA to measure autoantibodies to Hsp90 and Hsp70 in SLE patients, healthy controls and patients with a variety of autoimmune rheumatic diseases. IgG and IgM antibodies were elevated in 26 and 35% of SLE patients, respectively. These results show autoantibodies to Hsp90 (but not Hsp70) are elevated in a significant proportion of patients with SLE (P < 0.025) compared to healthy controls; and that those with raised antibody levels were more likely to have renal disease and a low C3 level (P < 0.02).

摘要

在大约25%的系统性红斑狼疮（SLE）患者外周血单个核细胞中，高度保守的90 kDa热休克蛋白（Hsp90）表达升高。关于使用复杂蛋白质印迹系统的先前方法检测抗Hsp90抗体的频率，已发表了相互矛盾的数据。我们现在描述一种酶联免疫吸附测定（ELISA）法，用于检测SLE患者、健康对照者以及患有各种自身免疫性风湿性疾病患者体内的抗Hsp90和抗Hsp70自身抗体。SLE患者中，IgG和IgM抗体升高的比例分别为26%和35%。这些结果表明，与健康对照者相比，相当一部分SLE患者体内抗Hsp90（而非抗Hsp70）自身抗体升高（P < 0.025）；并且抗体水平升高的患者更有可能患有肾脏疾病且C3水平较低（P < 0.02）。