人组织和血浆中免疫反应性肾上腺髓质素的分布与特征

Distribution and characterization of immunoreactive adrenomedullin in human tissue and plasma.

作者信息

Ichiki Y, Kitamura K, Kangawa K, Kawamoto M, Matsuo H, Eto T

机构信息

First Department of Internal Medicine, Miyazaki Medical College, Japan.

出版信息

FEBS Lett. 1994 Jan 24;338(1):6-10. doi: 10.1016/0014-5793(94)80106-1.

DOI:10.1016/0014-5793(94)80106-1

PMID:8307158

Abstract

A specific and sensitive radioimmunoassay for human adrenomedullin has been developed and distribution and characterization of immunoreactive adrenomedullin in human tissue were investigated. The radioimmunoassay specifically recognizes its carboxyterminal region and half maximal inhibition of binding of radioiodinated adrenomedullin(40-52)NH2 was observed at 11 fmol/tube. Immunoreactive adrenomedullin was abundant in adrenal medulla (47.7 +/- 26.1 fmol/mg, mean +/- S.D.) and was ubiquitously found in all tissue examined. The mean plasma concentration of adrenomedullin in three normal individuals was 17.2 +/- 6.4 pg/ml (mean +/- S.D.). By analysis with reverse-phase high-performance liquid chromatography coupled with the radioimmunoassay, most immunoreactive adrenomedullin in the adrenal medulla, atrium and lung was found to be adrenomedullin(1-52)NH2.

摘要

已开发出一种针对人肾上腺髓质素的特异性和灵敏的放射免疫测定法，并对人组织中免疫反应性肾上腺髓质素的分布和特性进行了研究。该放射免疫测定法能特异性识别其羧基末端区域，在11 fmol/管时观察到碘化肾上腺髓质素（40 - 52）NH2结合的半数最大抑制。免疫反应性肾上腺髓质素在肾上腺髓质中含量丰富（47.7±26.1 fmol/mg，平均值±标准差），并且在所检查的所有组织中均普遍存在。三名正常个体的肾上腺髓质素平均血浆浓度为17.2±6.4 pg/ml（平均值±标准差）。通过反相高效液相色谱结合放射免疫测定法分析，发现肾上腺髓质、心房和肺中大多数免疫反应性肾上腺髓质素为肾上腺髓质素（1 - 52）NH2。