Ichiki Y, Kitamura K, Kangawa K, Kawamoto M, Matsuo H, Eto T
First Department of Internal Medicine, Miyazaki Medical College.
J Biochem. 1995 Oct;118(4):765-70. doi: 10.1093/oxfordjournals.jbchem.a124978.
Using a sensitive radioimmunoassay which recognizes the carboxy-terminal region of porcine adrenomedullin, the distribution of immunoreactive adrenomedullin was widely distributed in porcine tissue, and its concentration was highest in adrenal medulla (126 +/- 32 fmol/mg wet tissue; mean +/- SD). Relatively high concentrations of immunoreactive adrenomedullin were found in the right atrium, lung, duodenum, and pituitary gland. The molecular form of immunoreactive adrenomedullin in all porcine tissues was found to be almost wholly porcine adrenomedullin itself by analysis with reverse-phase high-performance liquid chromatography coupled with radioimmunoassay. Minor molecular forms of immunoreactive adrenomedullin were found in the porcine duodenum. These were isolated and identified as adrenomedullin [26-52] and adrenomedullin [34-52].
采用一种能识别猪肾上腺髓质素羧基末端区域的灵敏放射免疫分析法，发现免疫反应性肾上腺髓质素在猪组织中广泛分布，其浓度在肾上腺髓质中最高（126±32 fmol/mg湿组织；均值±标准差）。在右心房、肺、十二指肠和垂体中发现了相对较高浓度的免疫反应性肾上腺髓质素。通过反相高效液相色谱结合放射免疫分析法分析发现，所有猪组织中免疫反应性肾上腺髓质素的分子形式几乎完全是猪肾上腺髓质素本身。在猪十二指肠中发现了免疫反应性肾上腺髓质素的次要分子形式。这些形式被分离并鉴定为肾上腺髓质素[26 - 52]和肾上腺髓质素[34 - 52]。
