Byrd W, Drobnis E Z, Kutteh W H, Marshburn P, Carr B R
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75205-9032.
Fertil Steril. 1994 Oct;62(4):850-6. doi: 10.1016/s0015-0282(16)57015-0.
To compare the pregnancy rates (PRs) after intrauterine insemination (IUI) with frozen-thawed donor sperm. Sperm were isolated after thawing using three different sperm preparation techniques: simple washing, Percoll density gradient, and Sephadex columns (SpermPrep Column; Fertility Technologies, Natick, MA).
Women (n = 98) were randomized upon entry into the program into one of three different sperm preparation methods. The same sperm preparation technique was used for the woman during subsequent cycles, if pregnancy did not occur. The study was stopped when > or = 75 treatment cycles for each group were completed for analysis of PRs per treatment cycle.
All patients were treated at our private care center at the University of Texas Southwestern Medical Center.
Patients entering this study were spontaneously ovulating women undergoing IUI with frozen donor sperm.
Pregnancy rate per cycle of timed IUI.
After 260 cycles of insemination, the PR per cycle was 19.1% with simple washing, 16.9% with Sephadex columns, and 11.4% with Percoll density gradient. Although these results were not statistically different, Percoll density gradient had a 40% lower PR per treatment cycle compared with simple washing. However, Percoll density gradient preparation of sperm resulted in a statistically significant increase in the motility, curvilinear velocity, straight line velocity, and the number of normal heads compared with the other two treatments.
Although Percoll density gradient separation of sperm results in a population of cells that is more motile and morphologically normal, this did not result in subsequent cycle fecundity. These data suggest that the reliance on the averaged values of motility, curvilinear velocity, straight line velocity and morphology may have little predictive value of the potential fertility of frozen-thawed sperm.
比较使用冷冻解冻供体精子进行宫腔内人工授精（IUI）后的妊娠率（PRs）。解冻后使用三种不同的精子制备技术分离精子：简单洗涤、Percoll密度梯度法和葡聚糖凝胶柱法（精子制备柱；生育技术公司，马萨诸塞州纳蒂克）。
98名女性在进入该项目时被随机分为三种不同精子制备方法中的一种。如果未怀孕，后续周期中该女性使用相同的精子制备技术。当每组完成≥75个治疗周期以分析每个治疗周期的PRs时，研究停止。
所有患者均在德克萨斯大学西南医学中心的私人护理中心接受治疗。
进入本研究的患者为使用冷冻供体精子进行IUI的自然排卵女性。
定时IUI每个周期的妊娠率。
在260个授精周期后，简单洗涤法每个周期的PR为19.1%，葡聚糖凝胶柱法为16.9%，Percoll密度梯度法为11.4%。尽管这些结果无统计学差异，但与简单洗涤相比，Percoll密度梯度法每个治疗周期的PR低40%。然而，与其他两种处理相比，Percoll密度梯度法制备的精子在活力、曲线速度、直线速度和正常头部数量方面有统计学显著增加。
尽管Percoll密度梯度法分离精子会产生活力更强、形态更正常的细胞群，但这并未导致后续周期的生育力提高。这些数据表明，依赖活力、曲线速度、直线速度和形态的平均值对冷冻解冻精子的潜在生育能力可能几乎没有预测价值。
