Lee J C, Chang J G
Department of Forensic Science, Central Police College, Taoyuan, Taiwan, ROC.
Forensic Sci Int. 1994 Jul 4;67(2):103-7. doi: 10.1016/0379-0738(94)90325-5.
The random amplified polymorphic DNA (RAPD) method was used to identify the species of forensic biological samples. Neither genomic DNA sequence nor two polymerase chain reaction (PCR) cycle programs is required. Only single 10-nt primer and one PCR program are used. We demonstrated that this method can be used to identify animals including bovine, goat, pig, dog, rat, rabbit, chicken, duck, and human by comparing their RAPD PCR fingerprints. This study provides a simple, fast and sensitive fingerprinting method in species identification for crime scene evidence or food products of endangered species.
随机扩增多态性DNA（RAPD）方法被用于鉴定法医物证样本的物种。既不需要基因组DNA序列，也不需要两个聚合酶链反应（PCR）循环程序。仅使用单个10核苷酸引物和一个PCR程序。我们通过比较动物的RAPD PCR指纹图谱证明，该方法可用于鉴定包括牛、山羊、猪、狗、大鼠、兔子、鸡、鸭和人类在内的动物。本研究为犯罪现场证据或濒危物种食品的物种鉴定提供了一种简单、快速且灵敏的指纹图谱方法。
