膜蛋白拓扑结构的基因融合分析：碱性磷酸酶和β-内酰胺酶融合的直接比较
Gene fusion analysis of membrane protein topology: a direct comparison of alkaline phosphatase and beta-lactamase fusions.
作者信息
Prinz W A, Beckwith J
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
J Bacteriol. 1994 Oct;176(20):6410-3. doi: 10.1128/jb.176.20.6410-6413.1994.
Abstract
To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to beta-lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent beta-lactamase fusions do not. This disparity may reflect differences in the folding of beta-lactamase and alkaline phosphatase in the cytoplasm.
摘要
为了比较两种分析膜蛋白拓扑结构的方法，许多与膜蛋白融合的碱性磷酸酶被转化为β-内酰胺酶融合蛋白。虽然一些位于膜蛋白细胞质环N端附近的碱性磷酸酶融合蛋白具有异常高的活性水平，但相应的β-内酰胺酶融合蛋白却没有。这种差异可能反映了β-内酰胺酶和碱性磷酸酶在细胞质中折叠方式的不同。
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本文引用的文献
1
The topological analysis of integral cytoplasmic membrane proteins.完整细胞质膜蛋白的拓扑分析
J Membr Biol. 1993 Feb;132(1):1-11. doi: 10.1007/BF00233047.
2
Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli.大肠杆菌细胞质膜中ExbB蛋白的拓扑结构。
J Biol Chem. 1993 Mar 15;268(8):6050-7.
3
The topology of the anchor subunit of dimethyl sulfoxide reductase of Escherichia coli.
J Biol Chem. 1993 Feb 15;268(5):3238-44.
4
Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions.使用最少数量的碱性磷酸酶融合蛋白分析膜蛋白的拓扑结构。
J Bacteriol. 1993 Jan;175(2):553-6. doi: 10.1128/jb.175.2.553-556.1993.
5
Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli.能使二硫键在大肠杆菌细胞质中形成的突变。
Science. 1993 Dec 10;262(5140):1744-7. doi: 10.1126/science.8259521.
6
Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli.改变大肠杆菌中碱性磷酸酶信号序列的突变。
J Bacteriol. 1983 Apr;154(1):366-74. doi: 10.1128/jb.154.1.366-374.1983.
7
Suppression of a signal sequence mutation by an amino acid substitution in the mature portion of the maltose-binding protein.麦芽糖结合蛋白成熟部分的氨基酸取代对信号序列突变的抑制作用。
J Bacteriol. 1987 May;169(5):1794-800. doi: 10.1128/jb.169.5.1794-1800.1987.
8
A vector for the construction of translational fusions to TEM beta-lactamase and the analysis of protein export signals and membrane protein topology.一种用于构建与TEMβ-内酰胺酶的翻译融合体以及分析蛋白质输出信号和膜蛋白拓扑结构的载体。
Gene. 1986;49(3):341-9. doi: 10.1016/0378-1119(86)90370-7.
9
Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltose-binding protein in E. coli.输出与成熟物种缺乏三级结构之间的相关性：大肠杆菌中麦芽糖结合蛋白的体内研究。
Cell. 1986 Sep 12;46(6):921-8. doi: 10.1016/0092-8674(86)90074-7.
10
A genetic approach to analyzing membrane protein topology.一种分析膜蛋白拓扑结构的遗传学方法。
Science. 1986 Sep 26;233(4771):1403-8. doi: 10.1126/science.3529391.
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