Giorgi J V, Ho H N, Hirji K, Chou C C, Hultin L E, O'Rourke S, Park L, Margolick J B, Ferbas J, Phair J P
Department of Medicine, University of California, Los Angeles (UCLA).
J Infect Dis. 1994 Oct;170(4):775-81. doi: 10.1093/infdis/170.4.775.
Subsets of activated CD8+ lymphocytes defined by membrane expression of the activation antigens HLA-DR and CD38 were counted by three-color flow cytometry in homosexual men who subsequently became seropositive for human immunodeficiency virus type 1 (HIV). Profound CD8+ cell activation was seen in all subjects at seroconversion and 6 and 12 months later. The HLA-DR+ CD38+ CD8+ cell population, which has potent direct HIV cytotoxic T cell activity, was markedly elevated at seroconversion in all subjects. In some men, these levels remained elevated throughout the first year of infection. During the next 5 years, these men had stable CD4+ cell levels, whereas the others did not. Long-term survivors (seropositive for 9 years, > 800 CD4+ cells/mm3) also had elevated levels of this subset, despite few other activated CD8+ cells. Thus, selective elevation of HLA-DR+ CD38- CD8+ cells was a marker of subsequent stable HIV disease.
通过三色流式细胞术对同性恋男性中由活化抗原HLA - DR和CD38的膜表达所定义的活化CD8 +淋巴细胞亚群进行计数，这些男性随后成为1型人类免疫缺陷病毒（HIV）血清阳性。在血清转化时以及6个月和12个月后，所有受试者均出现了深刻的CD8 +细胞活化。具有强大直接HIV细胞毒性T细胞活性的HLA - DR + CD38 + CD8 +细胞群体在所有受试者血清转化时均显著升高。在一些男性中，这些水平在感染的第一年一直保持升高。在接下来的5年中，这些男性的CD4 +细胞水平保持稳定，而其他男性则不然。长期存活者（血清阳性9年，CD4 +细胞> 800个/mm3）尽管其他活化CD8 +细胞很少，但该亚群水平也升高。因此，HLA - DR + CD38 - CD8 +细胞的选择性升高是随后HIV疾病稳定的一个标志。
