Toriumi Y, Samuel I, Wilcockson D P, Joehl R J
Surgical Service, Veterans Affairs Lakeside Medical Center, Chicago, IL.
Lab Anim Sci. 1994 Jun;44(3):270-3.
Diversion and recirculation of bile and pancreatic juice in rats are done in studies of pancreatic exocrine secretion. Previously, the modified Bollman cage was used to restrain rats with bile and pancreatic fistulas. To mimic physiologic conditions as closely as possible and to develop a more humane model, we designed a partial-restraint tethering system to study pancreatic exocrine secretion. Eight rats were prepared with biliary pancreatic, and duodenal fistulas, of which five were given enteral supplements via a gastric fistula and three were given saline supplements via a jugular venous line. Catheters exited at the nape of the neck and passed through the hollow of a cable coil that tethered the rat. On the fourth postoperative day pancreatic juice flow and protein output were studied. The tethering system allowed grooming, feeding, and ample mobility. This model of the tethered pancreatic fistula rat is a more human model for studies of pancreatic exocrine secretion in conscious rats, compared with the Bollman cage system of near total restraint.
在胰腺外分泌研究中，会对大鼠进行胆汁和胰液的改道及再循环操作。此前，改良的博尔曼笼被用于约束患有胆汁和胰瘘的大鼠。为了尽可能模拟生理状况并开发出更人道的模型，我们设计了一种部分约束的系留系统来研究胰腺外分泌。八只大鼠制备了胆管、胰管和十二指肠瘘，其中五只通过胃瘘给予肠内补充剂，三只通过颈静脉导管给予生理盐水补充剂。导管从颈部后方引出，穿过系住大鼠的电缆线圈的中空部分。术后第四天，对胰液流量和蛋白质输出进行了研究。该系留系统允许大鼠梳理毛发、进食并具有充足的活动能力。与几乎完全约束的博尔曼笼系统相比，这种系留胰瘘大鼠模型是一种更人道的用于清醒大鼠胰腺外分泌研究的模型。
