Watkins W B
Cell Tissue Res. 1976 Dec 3;175(2):165-81. doi: 10.1007/BF00232077.
Antibodies raised against porcine neurophysin-I and porcine neurophysin-II using an injection regime in rabbits over a short time period, were used to localize neurophysin-I and neurophysin-II in hypothalamic neurosecretory elements of the domestic pig. In transverse section, neurophysin-II containing cells were more abundant in the dorsal medial region of the rostral supraoptic nucleus (SON) as compared with the distribution of neurophysin-I neurons. The main bulk of the cells of the SON were heavily stained for neurophysin-I with neurophysin-II containing cells positioned dorsal from the edge of the optic chiasma. Neurosecretory cells of the SON as seen in sagittal section also showed a differential staining for neurophysins-I and -II. Rostral regions of the pig paraventricular nucleus (PVN) contained magnocellular elements near the third ventricle which were stained predominantly for neurophysin-II. In regions corresponding to the caudal PVN there appeared two populations of neurosecretory neurons: (a) an area of cells adjacent to the third ventricle which contained neurophysin-II antigen and (b) a group of densely populated cells in the dorsal-lateral region which was stained for neurophysin-I. The results support the existence in the pig of at least two distinct populations of neurosecretory neurons corresponding to the neurophysin-I and neurophysin-II neurosecretory system.
在短时间内通过给兔子注射的方式制备了抗猪神经垂体素 -I 和抗猪神经垂体素 -II 的抗体，并用于在家猪下丘脑神经分泌元件中定位神经垂体素 -I 和神经垂体素 -II。在横切面上，与神经垂体素 -I 神经元的分布相比，含神经垂体素 -II 的细胞在视上核（SON）嘴侧的背内侧区域更为丰富。SON 的大部分细胞被神经垂体素 -I 重度染色，含神经垂体素 -II 的细胞位于视交叉边缘的背侧。矢状面观察到的 SON 神经分泌细胞对神经垂体素 -I 和 -II 也有不同的染色。猪室旁核（PVN）的嘴侧区域在第三脑室附近含有大细胞成分，主要被神经垂体素 -II 染色。在对应于 PVN 尾侧的区域出现了两类神经分泌神经元：（a）靠近第三脑室的一群细胞，含有神经垂体素 -II 抗原；（b）背外侧区域一群密集的细胞，被神经垂体素 -I 染色。这些结果支持猪体内至少存在两类不同的神经分泌神经元群体，分别对应神经垂体素 -I 和神经垂体素 -II 神经分泌系统。
