大鼠肝脏中与黄素腺嘌呤二核苷酸（FAD）相连的甘油-3-磷酸脱氢酶的cDNA克隆及其受甲状腺激素的调控
Cloning of a cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones.
作者信息
Müller S, Seitz H J
机构信息
Institut für Physiologische Chemie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
出版信息
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10581-5. doi: 10.1073/pnas.91.22.10581.
A full-length 2.4-kb cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) was cloned from rat liver using PCR techniques. The cloned gene encodes a protein of 727 amino acids. The calculated molecular mass of 80,898 Da is higher than the apparent molecular mass observed by SDS/PAGE (74,000 Da) of the purified enzyme. This result indicates that the enzyme is synthesized as a precursor with a putative mitochondrial signal sequence. mRNA for this gene was detected in liver, heart, muscle, brain, testes, and pancreas. With the exception of testes, basal expression levels were very low in all tissues examined. However, application of thyroid hormones led to a 10- to 15-fold increase in liver glycerol-3-phosphate dehydrogenase mRNA, whereas hypothyroidism further decreased the mRNA level.
利用聚合酶链反应（PCR）技术从大鼠肝脏中克隆出了与黄素腺嘌呤二核苷酸（FAD）相连的甘油-3-磷酸脱氢酶（EC 1.1.99.5）的全长2.4千碱基（kb）的互补脱氧核糖核酸（cDNA）。克隆出的基因编码一种由727个氨基酸组成的蛋白质。计算得出的分子量为80,898道尔顿（Da），高于通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS/PAGE）观察到的纯化酶的表观分子量（74,000 Da）。这一结果表明该酶是以带有假定线粒体信号序列的前体形式合成的。在肝脏、心脏、肌肉、大脑、睾丸和胰腺中检测到了该基因的信使核糖核酸（mRNA）。除睾丸外，在所检测的所有组织中基础表达水平都非常低。然而，应用甲状腺激素会使肝脏中甘油-3-磷酸脱氢酶mRNA增加10至15倍，而甲状腺功能减退则会使mRNA水平进一步降低。
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