Takakuwa T, Endo S, Nakae H, Kikuchi M, Baba N, Inada K, Yoshida M
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Res Commun Chem Pathol Pharmacol. 1994 Jun;84(3):291-300.
We measured serum levels of endotoxin, cytokines, and eicosanoids and investigated their relationship to serum complement levels in patients with sepsis. Serum endotoxin (Et) levels (5.3 +/- 2.4 pg/ml) were within the normal range, but levels of tumor necrosis factor-alpha (TNF-alpha, 114 +/- 104.94 pg/ml), interleukin 6 (IL-6, 86.7 +/- 50.9 pg/ml), interleukin 8 (IL-8, 86.8 +/- 49.7 pg/ml), type-II phospholipase A2 (type II PLA2, 211.3 +/- 193.9 ng/ml), leukotriene B4 (LTB4, 88.7 +/- 27.2 pg/ml), thromboxane B2 (TXB2, 58.7 +/- 50.9 pg/ml) and 6-keto-prostaglandin F1 alpha (PGF1 alpha, 21.0 +/- 11.0 pg/ml) levels were above normal. Levels of C3a (1088.4 +/- 83.8.7 ng/ml) and C4a (1951.5 +/- 1697.8 ng/ml) were also above normal; C3 (66.0 +/- 25.6 mg/dl) and C4 (23.6 +/- 5.3 mg/dl) were within the normal range, and C5a was lower than the detectable limit in all but one of the subjects. Serum TNF-alpha was significantly correlated with C3a (p < 0.001). Serum IL-6 had a significant negative correlation with C3 (p = 0.002) and C4 (p = 0.010). Type II PLA2 was significantly correlated with C3a (p < 0.001). There were no significant correlations between serum Et or IL-8 and serum C3, C4, C3a or C4a. Our findings suggest that increased levels of TNF-alpha, IL-6, and Type II PLA/ in patients with sepsis contribute to activation of the complement system.
我们检测了脓毒症患者血清中的内毒素、细胞因子和类花生酸水平，并研究了它们与血清补体水平的关系。血清内毒素（Et）水平（5.3±2.4 pg/ml）在正常范围内，但肿瘤坏死因子-α（TNF-α，114±104.94 pg/ml）、白细胞介素6（IL-6，86.7±50.9 pg/ml）、白细胞介素8（IL-8，86.8±49.7 pg/ml）、II型磷脂酶A2（II型PLA2，211.3±193.9 ng/ml）、白三烯B4（LTB4，88.7±27.2 pg/ml）、血栓素B2（TXB2，58.7±50.9 pg/ml）和6-酮-前列腺素F1α（PGF1α，21.0±11.0 pg/ml）水平高于正常。C3a（1088.4±83.8.7 ng/ml）和C4a（1951.5±1697.8 ng/ml）水平也高于正常；C3（66.0±25.6 mg/dl）和C4（23.6±5.3 mg/dl）在正常范围内，除1名受试者外，所有受试者的C5a均低于可检测限。血清TNF-α与C3a显著相关（p<0.001）。血清IL-6与C3（p = 0.002）和C4（p = 0.010）呈显著负相关。II型PLA2与C3a显著相关（p<0.001）。血清Et或IL-8与血清C3、C4、C3a或C4a之间无显著相关性。我们的研究结果表明，脓毒症患者TNF-α、IL-6和II型PLA水平升高有助于补体系统的激活。
