Takakuwa T, Endo S, Nakae H, Suzuki T, Inada K, Yoshida M, Ogawa M, Uchida K
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Res Commun Chem Pathol Pharmacol. 1994 Jun;84(3):271-81.
We determined the plasma levels of type-II phospholipase A2 (type II PLA2), platelet-activating factor acetylhydrolase (PAFAH) leukotriene B4 (LTB4) and of several complements (C3a, C4a, and C5a), which are considered to be among the cytokines and eicosanoids involved in vascular endothelial disorders and that vary in concentration during sepsis. We investigated the relationship between those levels and those of ET-1 and TM levels in plasma. Plasma levels of type II PLA2, PAFAH, LTB4, C3a, C4a, ET-1, and TM at the time that sepsis was diagnosed in 30 patients were 218.3 +/- 179.9 ng/ml, 23.92 +/- 9.66 nmol/min/ml, 90.35 +/- 31.49 pg/ml, 838.73 +/- 2.30 pg/ml, 1951.46 +/- 1697.78 pg/ml, 6.98 +/- 4.08 pg/ml and 7.80 +/- 3.34 ng/ml, respectively. The C5a plasma level was below the limit of detection in all cases. There were significant correlations between type II PLA2 and ET-1 plasma levels (r = 0.39, p = 0.032) and C3a and ET-1 plasma levels (r = 0.60, p = 0.03). There were also significant correlations between type II PLA2 and TM levels in plasma (r = 0.76, p = 0.0017), PAFAH and TM plasma levels (r = 0.53, p = 0.037), LTB4 and TM plasma levels (r = 0.46, p = 0.016) and C4a and TM plasma levels (r = 0.58, p = 0.037). Results suggest that the elevation of type II PLA2, PAFAH, LTB4 and complement in plasma is involved in vascular endothelial disorders in patients with sepsis.
我们测定了II型磷脂酶A2（II型PLA2）、血小板活化因子乙酰水解酶（PAFAH）、白三烯B4（LTB4）以及几种补体（C3a、C4a和C5a）的血浆水平，这些物质被认为是参与血管内皮疾病的细胞因子和类花生酸，且在脓毒症期间其浓度会发生变化。我们研究了这些水平与血浆中内皮素-1（ET-1）水平以及血栓调节蛋白（TM）水平之间的关系。30例患者脓毒症诊断时的II型PLA2、PAFAH、LTB4、C3a、C4a、ET-1和TM的血浆水平分别为218.3±179.9 ng/ml、23.92±9.66 nmol/分钟/ml、90.35±31.49 pg/ml、838.73±2.30 pg/ml、1951.46±1697.78 pg/ml、6.98±4.08 pg/ml和7.80±3.34 ng/ml。所有病例的C5a血浆水平均低于检测限。II型PLA2与ET-1血浆水平之间存在显著相关性（r = 0.39，p = 0.032），C3a与ET-1血浆水平之间也存在显著相关性（r = 0.60，p = 0.03）。II型PLA2与血浆中TM水平之间、PAFAH与血浆中TM水平之间、LTB4与血浆中TM水平之间以及C4a与血浆中TM水平之间也存在显著相关性（r分别为0.76，p = 0.0017；r = 0.53，p = 0.037；r = 0.46，p = 0.016；r = 0.58，p = 0.037）。结果表明，血浆中II型PLA2、PAFAH、LTB4和补体水平的升高与脓毒症患者的血管内皮疾病有关。
