Nakae H, Endo S, Inada K, Yamashita H, Yamada Y, Takakuwa T, Kasai T, Ogawa M, Uchida K
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Burns. 1995 Sep;21(6):422-6. doi: 10.1016/0305-4179(95)00022-4.
The plasma concentrations of type II phospholipase A2 (type II PLA2) and eicosanoids, such as leukotriene B4 (LTB4), 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), and thromboxane B2 (TXB2), were determined by radioimmunoassay in 23 patients with burns covering at least 20 per cent of their body surface. Cytokines such as tumour necrosis factor-alpha (TNF-alpha), interleukin 6 (IL-6) and interleukin 8 (IL-8) were determined by enzyme-immunosorbent assay. There was no increase in type II PLA2 concentration in the early stage of burns, but an increase in type II PLA2 concentration was triggered by infection (P < 0.0001). The level of type II PLA2 was significantly higher in the non-surviving group than in the surviving group (P = 0.0006), suggesting that it reflects the severity of the disease. There was a significant correlation between the maximum level of type II PLA2 and TNF-alpha (r = 0.6346, P = 0.0011). There was a significant correlation between the maximum level of type II PLA2 and the accompanying plasma concentrations of LTB4, 6-keto-PGF1 alpha, and TXB2 throughout the observation period (r = 0.4814, P = 0.0200; r = 0.5943, P = 0.0028; r = 0.4368, P = 0.0372 respectively). Plasma levels of LTB4, and TXB2 were significantly higher in the burn patients who died than in those who survived (P = 0.0493; P = 0.0493 respectively).
采用放射免疫分析法测定了23例烧伤面积至少达体表面积20%的患者血浆中II型磷脂酶A2（II型PLA2）以及类花生酸的浓度，如白三烯B4（LTB4）、6-酮-前列腺素F1α（6-酮-PGF1α）和血栓素B2（TXB2）。采用酶联免疫吸附测定法测定了细胞因子，如肿瘤坏死因子-α（TNF-α）、白细胞介素6（IL-6）和白细胞介素8（IL-8）。烧伤早期II型PLA2浓度没有升高，但感染会引发II型PLA2浓度升高（P<0.0001）。非存活组的II型PLA2水平显著高于存活组（P=0.0006），这表明它反映了疾病的严重程度。II型PLA2的最高水平与TNF-α之间存在显著相关性（r=0.6346，P=0.0011）。在整个观察期内，II型PLA2的最高水平与伴随的血浆LTB4、6-酮-PGF1α和TXB2浓度之间存在显著相关性（分别为r=0.4814，P=0.0200；r=0.5943，P=0.0028；r=0.4368，P=0.0372）。死亡烧伤患者的血浆LTB4和TXB2水平显著高于存活患者（分别为P=0.0493；P=0.0493）。
