Bødker A, Andersson K E, Batra S, Juhl B R, Meyhoff H H
Department of Urology, Glostrup Hospital, University of Copenhagen, Denmark.
Scand J Urol Nephrol. 1994 Jun;28(2):113-8. doi: 10.3109/00365599409180485.
The influence of castration on estrogen receptor (ER) expression in the male rabbit prostate and urethra, was investigated immunohistochemically and biochemically in 18 mature male, rabbits 14 days after bilateral orchiectomy, and in 15 controls. Immunohistochemical scores of ER were increased significantly in the prostatic stroma and the urethral submucosa (p < 0.05 Mann-Whitney), while the increase in the urothelium was not significant. Biochemically, the ER content was 87.4 +/- 42.6 f mol/mg protein in the normal prostate, and 190.0 +/- 28.0 f mol/mg protein following castration. The corresponding values for the posterior urethra were 228.3 +/- 43.2 f mol/mg protein and 354.9 +/- 60.1 f mol/mg protein. In both cases the increase was not significant. We conclude, that ERs in the prostate and the urethra of the male rabbit can be modified by hormonal manipulation. ERs may therefore be functionally active and not rudimentary.
通过免疫组织化学和生物化学方法，对18只成熟雄性兔子在双侧睾丸切除术后14天以及15只对照兔子进行研究，以探讨去势对雄性兔前列腺和尿道中雌激素受体（ER）表达的影响。ER的免疫组织化学评分在前列腺基质和尿道黏膜下层显著增加（曼-惠特尼检验，p < 0.05），而在尿路上皮中的增加不显著。生物化学分析显示，正常前列腺中ER含量为87.4±42.6 fmol/mg蛋白质，去势后为190.0±28.0 fmol/mg蛋白质。后尿道的相应值分别为228.3±43.2 fmol/mg蛋白质和354.9±60.1 fmol/mg蛋白质。在这两种情况下，增加均不显著。我们得出结论，雄性兔前列腺和尿道中的ER可通过激素调控发生改变。因此，ER可能具有功能活性而非发育不全。
