Quigley M E, Englyst H N
Dunn Clinical Nutrition Centre, Addenbrookes Hospital Site, Cambridge, UK.
Analyst. 1994 Jul;119(7):1511-8. doi: 10.1039/an9941901511.
A technique is described for measurement of the uronic acid constituents of non-starch polysaccharides (NSP) by high-performance liquid chromatography with pulsed amperometric detection using two sets of hydrolysis conditions. In one set of hydrolysis conditions the uronic acid-containing polymers are hydrolysed by prolonged treatment with sulfuric acid only; in the other, the hydrolysate obtained in the Englyst procedure for the measurement of dietary fibre as NSP is buffered to between pH 3.5 and 4 and the uronic acid-containing polymers present are depolymerized enzymically. The conditions for acid hydrolysis only or acid and enzymic hydrolysis of uronic acid-containing polymers optimized for a range of fruit, vegetable and cereal products. Values obtained for total uronic acids are very similar using either hydrolysis procedure, and are in good agreement with values obtained using the spectrophotometric assay as used in the Englyst procedure.
本文描述了一种利用高效液相色谱和脉冲安培检测法，通过两组水解条件来测定非淀粉多糖（NSP）中糖醛酸成分的技术。在一组水解条件下，仅用硫酸长时间处理使含糖醛酸的聚合物水解；在另一组条件下，将Englyst法测定膳食纤维（作为NSP）时得到的水解产物缓冲至pH 3.5至4之间，使存在的含糖醛酸聚合物进行酶解聚。针对一系列水果、蔬菜和谷物产品，优化了仅酸水解或含糖醛酸聚合物的酸和酶水解条件。使用任何一种水解方法得到的总糖醛酸值都非常相似，并且与Englyst法中使用的分光光度法测定的值高度一致。
