[利用大肠杆菌限制性内切酶EcoR I和BAM I对人6型腺病毒DNA进行片段化]
[Fragmentation of human adenovirus type 6 DNA by means of restrictase of E. co R I and BAM I].
作者信息
Naroditskiĭ B S, Karamov E V, Chaplygina N A, Zavizion B A, Tikhonenko T I
出版信息
Vopr Virusol. 1976 Sep-Oct(5):539-44.
The effect of restricting endonucleases of E. co RI and BAMI on DNA of human adenovirus type 6 was studied. E. co RI and BAMI restrictases cleave virus DNA into 4 fragments each. The molecular weight of E. co RI fragments was: A--17.5X10(6), B--3.63X10(6), C--1.56X10(6), D--1.05X10(6), those of BAMI fragments: A--9.7X10(6), B--7X10(6), C--4.09X10(6), D--3.1X10(6). By means of terminal nucleotidyltransferase and the procedure of partial DNA hydrolysis, the alteration of fragments obtained by the effect of E. co RI enzyme in adenovirus type 6 genome was determined and found to be A--D--C--B.
研究了大肠杆菌限制性内切酶Eco RI和Bam HI对人6型腺病毒DNA的作用。Eco RI和Bam HI限制性内切酶分别将病毒DNA切割成4个片段。Eco RI片段的分子量为：A—17.5×10⁶，B—3.63×10⁶，C—1.56×10⁶，D—1.05×10⁶；Bam HI片段的分子量为：A—9.7×10⁶，B—7×10⁶，C—4.09×10⁶，D—3.1×10⁶。借助末端核苷酸转移酶和部分DNA水解程序，确定了6型腺病毒基因组中Eco RI酶作用产生的片段的排列顺序为A—D—C—B。
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