Olsen M A, Root P P
Department of Pathology, Creighton University School of Medicine, Omaha, Nebraska.
Diagn Microbiol Infect Dis. 1994 May;19(1):19-24. doi: 10.1016/0732-8893(94)90046-9.
Serum specimens from 246 women of childbearing age were tested for immunoglobulin G (IgG) antibodies to Toxoplasma gondii by four different commercial assays: Abbott IMx microparticle enzyme immunoassay (EIA), Mercia Toxo-G EIA, Bartels Prima EIA, and bioMerieux Vitek (VIDAS) enzyme-linked fluorescent assay. A total of 27 specimens were initially positive with all four assays, 202 specimens were negative, and 17 specimens were discrepant (disagreement among the assays). After repeating tests for the 17 discrepant samples, five resolved (one was positive and four were negative). The 12 remaining discrepant samples were tested by an indirect fluorescent antibody (IFA) assay for Toxoplasma IgG antibodies; five specimens were positive for Toxoplasma IgG by IFA. The resolved sensitivities of the various kits ranged from 88% (bioMerieux VIDAS) to 94% (Abbott IMx), and the specificities were all 98%-99%. These results show that the four serologic tests used for detection of Toxoplasma IgG give very similar results and can all be readily used by clinical laboratories for screening purposes.
采用四种不同的商业检测方法，对246名育龄妇女的血清标本进行了抗弓形虫免疫球蛋白G（IgG）抗体检测：雅培IMx微粒酶免疫测定法（EIA）、默西亚弓形虫G EIA、巴特尔斯拉伊玛EIA和生物梅里埃Vitek（VIDAS）酶联荧光测定法。最初，所有四种检测方法均呈阳性的标本有27份，阴性标本有202份，17份标本结果不一致（检测方法之间存在分歧）。对这17份结果不一致的样本重复检测后，5份结果得到解决（1份为阳性，4份为阴性）。对其余12份结果不一致的样本进行了弓形虫IgG抗体间接荧光抗体（IFA）检测；其中5份标本IFA检测显示弓形虫IgG呈阳性。各种试剂盒的灵敏度从88%（生物梅里埃VIDAS）到94%（雅培IMx）不等，特异性均为98%-99%。这些结果表明，用于检测弓形虫IgG的四种血清学检测方法结果非常相似，临床实验室均可方便地用于筛查目的。
