Miller J, Sande M A, Gwaltney J M, Hendley J O
J Clin Microbiol. 1978 May;7(5):459-62. doi: 10.1128/jcm.7.5.459-462.1978.
Pneumococcal polysaccharide was detected by counterimmunoelectrophoresis in the sputum of 20 of 26 (77%) adults with community-acquired pneumonia and a positive sputum culture for Streptococcus pneumoniae. The test was negative in 29 pneumonia patients with negative sputum culture for S. pneumoniae. Pneumococcal antigen was also detected in the sputum of six of nine adults with chronic bronchitis and a positive sputum culture, but was not detected in expectorated respiratory secretions of 22 pneumococcal carriers with colds. Pneumococcal antigen could also be detected in sputum by immunodiffusion; antigen titers varied from 1:2 to 1:256. These results strongly suggest that the detection of pneumococcal antigen in respiratory tract secretions indicates infection caused by S. pneumoniae.
采用对流免疫电泳法，在26例社区获得性肺炎且痰培养肺炎链球菌阳性的成人患者中，有20例（77%）痰液检测到肺炎球菌多糖。29例痰培养肺炎链球菌阴性的肺炎患者检测结果为阴性。9例慢性支气管炎且痰培养阳性的成人患者中，有6例痰液检测到肺炎球菌抗原，但22例感冒的肺炎球菌携带者咳出的呼吸道分泌物中未检测到。也可通过免疫扩散法在痰液中检测到肺炎球菌抗原；抗原滴度从1:2到1:256不等。这些结果有力地表明，呼吸道分泌物中肺炎球菌抗原的检测表明存在肺炎链球菌感染。
