Manos M M, Waldman J, Zhang T Y, Greer C E, Eichinger G, Schiffman M H, Wheeler C M
Department of Infectious Diseases, Cetus Corp., Emeryville.
J Infect Dis. 1994 Nov;170(5):1096-9. doi: 10.1093/infdis/170.5.1096.
Polymerase chain reaction (PCR)-based genital human papillomavirus (HPV) detection methods that use consensus primers have enabled the broad-spectrum detection of most characterized HPV types. In addition, these techniques have allowed the identification of potentially novel viral sequences in clinical specimens. These methods were used to determine the partial L1 nucleotide sequence (the region generated by L1 consensus primers MY09 and MY11) of four novel viruses. The prevalence of these viruses in cytologically normal and dysplastic cervical specimens and in invasive cervical cancer was also determined. The partial DNA sequences of W13B (MM4), PAP291 (MM7), PAP155 (MM8), and PAP238a (MM9) are most similar to HPV-51, -61, -61, and -34, respectively. Prevalence studies suggest that W13B and PAP238a are cancer-associated, while PAP155 and PAP291 appear to be lower-risk viruses.
使用共有引物的基于聚合酶链反应（PCR）的生殖器人乳头瘤病毒（HPV）检测方法，能够对大多数已明确特征的HPV类型进行广谱检测。此外，这些技术还能鉴定临床标本中潜在的新型病毒序列。这些方法被用于确定4种新型病毒的部分L1核苷酸序列（由L1共有引物MY09和MY11扩增的区域）。还测定了这些病毒在细胞学正常和发育异常的宫颈标本以及浸润性宫颈癌中的流行情况。W13B（MM4）、PAP291（MM7）、PAP155（MM8）和PAP238a（MM9）的部分DNA序列分别与HPV-51、-61、-61和-34最为相似。流行率研究表明，W13B和PAP238a与癌症相关，而PAP155和PAP291似乎是低风险病毒。
