Mabruk M J, Flint S R, Toner M, Balluz I, Coleman D, Sullivan D, Atkins G J
J Oral Pathol Med. 1994 Aug;23(7):302-8. doi: 10.1111/j.1600-0714.1994.tb00066.x.
The definitive diagnosis of oral hairy leukoplakia (OHL) demands that Epstein-Barr virus (EBV) is demonstrated in the lesional tissue, since the histopathological features on conventional light microscopy are not pathognomonic. We have investigated the possible use of polymerase chain reaction (PCR) technology in reaching a definitive diagnosis of this lesion by its application to ten biopsy specimens with definitive diagnoses of OHL determined by in situ hybridization. EBV DNA was demonstrated by PCR in all ten OHL biopsy specimens analysed, and none of ten control specimens. Furthermore, we have investigated the role of PCR in analysis of exfoliative cytology samples collected from the lateral border of the tongue by a minimally-invasive scraping technique. EBV DNA was not only detected in all OHL lesional scrapings but also in more than one-third of healthy controls, due to viral presence in saliva at the time of sampling. In this application, the highly sensitive PCR technique has low specificity and cannot be recommended.
口腔毛状白斑（OHL）的确诊需要在病变组织中检测到爱泼斯坦-巴尔病毒（EBV），因为传统光学显微镜下的组织病理学特征并无特异性。我们研究了聚合酶链反应（PCR）技术在该病变确诊中的应用可能性，将其应用于10例经原位杂交确诊为OHL的活检标本。在分析的所有10例OHL活检标本中，通过PCR均检测到EBV DNA，而10例对照标本均未检测到。此外，我们还研究了PCR在通过微创刮取技术从舌外侧缘采集的脱落细胞学样本分析中的作用。不仅在所有OHL病变刮片中检测到EBV DNA，而且在超过三分之一的健康对照中也检测到，这是由于采样时唾液中存在病毒。在这种应用中，高灵敏度的PCR技术特异性较低，因此不推荐使用。
