Departamento de Clínica e Odontologia Preventiva, Universidade Federal de Pernambuco, Oral Medicine Unit, Recife, PE, Brazil.
Clin Oral Investig. 2012 Apr;16(2):659-64. doi: 10.1007/s00784-011-0548-5. Epub 2011 Mar 29.
The purpose of this study is to determine the frequency of EBV and CMV DNA detection in saliva of HIV infected and non-HIV individuals and their siblings. The study group comprised 240 individuals. Group 1 comprised of 40 HIV-infected patients, group 2 40 non-HIV individuals, group 3 two siblings for each patient from group 1 (n = 80), and group 4 two siblings for each individual from group 2 (n = 80). Non-stimulated whole saliva was collected, DNA was extracted, and amplification was performed using a nested PCR protocol. EBV and CMV DNA was detected in 7/40 (17.5%) and 5/40 (12.5%) individuals from group 1, 8/40 (20%) and 3/40 (7.5%) from group 2, 11/80 (13.8%) and 2/80 (2.5%) from group 3, and 8/80 (10%) and 1/80 (1.3%) from group 4, respectively. Five (71.4%) out of seven HIV/EBV coinfected individuals of group 1 had a relative also infected with EBV (OR = 11.25, CI [1.75-72.5], p = 0.011). Regarding group 2, among the eight non-HIV and EBV-infected individuals, three (37.5%) had a relative also positive to EBV (p = 0.320). No individual HIV/CMV coinfected had a relative CMV infected (p = 1.00). Also, only one non-HIV and CMV-infected individual had a relative also positive to CMV (p = 0.075). EBV and CMV DNA was detected mainly in those who had HIV viral load counts <400/mL (71%, p = 0.2 and 100%, p = 1, respectively) and those who had CD4 T cells counts between 200 and 400/mm(3) (57%, p = 0.544 and 60%, p = 0.249, respectively). HIV-infected individuals and healthy controls showed a similar frequency of viral DNA detection. EBV DNA was significantly amplified in saliva of household members of HIV/EBV coinfected individuals.
本研究旨在确定 EBV 和 CMV DNA 在 HIV 感染者、非 HIV 感染者及其兄弟姐妹唾液中的检测频率。研究组包括 240 名个体。第 1 组包括 40 名 HIV 感染者，第 2 组包括 40 名非 HIV 感染者，第 3 组为第 1 组每位患者的 2 个兄弟姐妹（共 80 人），第 4 组为第 2 组每位个体的 2 个兄弟姐妹（共 80 人）。采集非刺激全唾液，提取 DNA，采用巢式 PCR 方案进行扩增。第 1 组有 7/40（17.5%）和 5/40（12.5%）个体检测到 EBV 和 CMV DNA，第 2 组有 8/40（20%）和 3/40（7.5%）个体检测到 EBV 和 CMV DNA，第 3 组有 11/80（13.8%）和 2/80（2.5%）个体检测到 EBV 和 CMV DNA，第 4 组有 8/80（10%）和 1/80（1.3%）个体检测到 EBV 和 CMV DNA。第 1 组中 7 名 HIV/EBV 合并感染的个体中有 5 名（71.4%）的亲属也感染了 EBV（OR=11.25，CI[1.75-72.5]，p=0.011）。关于第 2 组，在 8 名非 HIV 和 EBV 感染者中，有 3 名（37.5%）的亲属也感染了 EBV（p=0.320）。没有 HIV/CMV 合并感染的个体有亲属感染 CMV（p=1.00）。此外，只有 1 名非 HIV 和 CMV 感染者的亲属也感染了 CMV（p=0.075）。EBV 和 CMV DNA 主要在那些 HIV 病毒载量<400/mL（分别为 71%，p=0.2 和 100%，p=1）和那些 CD4 T 细胞计数在 200 和 400/mm3 之间（分别为 57%，p=0.544 和 60%，p=0.249）的个体中检测到。HIV 感染者和健康对照组的病毒 DNA 检测频率相似。EBV DNA 在 HIV/EBV 合并感染个体的家庭成员唾液中显著扩增。
