Yalcin S, Kuratsune H, Yamaguchi K, Kitani T, Yamanishi K
Department of Virology, Osaka University Medical School, Japan.
Microbiol Immunol. 1994;38(7):587-90. doi: 10.1111/j.1348-0421.1994.tb01827.x.
Peripheral blood mononuclear cells collected from 13 patients with chronic fatigue syndrome and 13 healthy controls were analyzed for the presence of human herpesvirus 6 (HHV-6) DNA by variant-specific polymerase chain reaction and dot blot hybridization. HHV-6 DNA was detected in 7 of 13 (53%) patients, and of those 7 patients, 4 were positive for HHV-6 variant A DNA and 3 were for variant B. No HHV-6 DNA was detected in the controls. Serum antibody titers to the late antigen and antibody prevalence to the early antigen of HHV-6 were significantly higher in the patient group. These results suggest active replication of HHV-6 in patients with chronic fatigue syndrome.
通过变异特异性聚合酶链反应和斑点印迹杂交，对13例慢性疲劳综合征患者和13名健康对照者采集的外周血单个核细胞进行人疱疹病毒6型（HHV-6）DNA检测。13例患者中有7例（53%）检测到HHV-6 DNA，其中4例HHV-6 A型DNA阳性，3例B型阳性。对照者中未检测到HHV-6 DNA。患者组中针对HHV-6晚期抗原的血清抗体滴度和针对早期抗原的抗体阳性率显著更高。这些结果提示慢性疲劳综合征患者中存在HHV-6的活跃复制。
