Fournier M, Gireau A, Boniface B, Ghevaert C, Lepelley P, Zandecki M, Goudemand J, Cosson A
Hôpital Claude Huriez, Laboratoire d'Hématologie, CHRU, Lille, France.
Nouv Rev Fr Hematol (1978). 1994 Jul;36 Suppl 2:S117-21.
Leucopenia patient follow-up remains, in terms of laboratory turnaround, a heavy workload due to the leucoconcentrations necessary for evaluating leucocytic formulas. We tested the CD 3500 with the objective of defining its' analytical performances and routine practice. 101 leucopenia samples (< 2.10(9)/l) procured from the onco-hematology department (adults and children) were studied during a 1 month period. The leucocytic formula obtained after leuconcentrations was our reference. The alarm sensitivity, as a whole, was of 97% for a 15.5% specificity. The correlation coefficients (Cell-Dyn/microscope) for polynuclears, lymphocytes and monocytes were respectively 0.889, 0.925 and 0.926. The correlation coefficients observed in both following subgroups: < or = 0.5.10(9)/l and > 0.5.10(9)/l were superposable. In 95% of the cases, the numeric value difference between the two methods attained a maximum of 21% for all neutrophils and lymphocytes and 13% for the monocytes. There was an excellent concordance between both methods for eosinophilia and basophils with confidence intervals of +/-8.8% and +/-2.2%. In practice, we feel that the use of a CD 3500 in post chemotherapy leucopenia, is perfectly adaptable and appreciated for leucocytic formulas, as well as a good exit for aplasia.
就实验室周转而言，由于评估白细胞分类所需的白细胞浓度，白细胞减少症患者的随访工作量仍然很大。我们对CD 3500进行了测试，目的是确定其分析性能和常规应用。在1个月的时间里，研究了从肿瘤血液科（成人和儿童）采集的101份白细胞减少症样本（<2×10⁹/L）。白细胞浓缩后获得的白细胞分类是我们的参考标准。总体而言，警报灵敏度为97%，特异性为15.5%。多核细胞、淋巴细胞和单核细胞的相关系数（Cell-Dyn/显微镜）分别为0.889、0.925和0.926。在以下两个亚组中观察到的相关系数：≤0.5×10⁹/L和>0.5×10⁹/L是可叠加的。在95%的病例中，两种方法之间的数值差异对于所有中性粒细胞和淋巴细胞最大达到21%，对于单核细胞为13%。嗜酸性粒细胞和嗜碱性粒细胞的两种方法之间有极好的一致性，置信区间为±8.8%和±2.2%。在实践中，我们认为在化疗后白细胞减少症中使用CD 3500对于白细胞分类非常适用且受到认可，并且对于再生障碍性贫血也是一个很好的解决方案。
