马铃薯线粒体中tRNA加工活性的表征与部分纯化
Characterization and partial purification of tRNA processing activities from potato mitochondria.
作者信息
Marchfelder A, Brennicke A
机构信息
Institut für Genbiologische Forschung GmbH, Berlin, Germany.
出版信息
Plant Physiol. 1994 Aug;105(4):1247-54. doi: 10.1104/pp.105.4.1247.
Abstract
In plant mitochondria, as in most other genetic systems, several enzymatic processing and modification steps are required to yield mature tRNAs from primary transcripts. Three of the enzymes involved, RNase P, 3'-processing activity, and tRNA nucleotidyl transferase, were identified in potato (Solanum tuberosum) mitochondria and have been separated by several purification steps. RNase P was partially purified, with only a few proteins detectable in active fractions after a final glycerol gradient step. A small RNA molecule present in fractions with RNase P activity contains the heptanucleotide conserved in the other known RNase P RNA sequences and may be a fragment of the RNA moiety of the plant mitochondrial RNase P.
摘要
与大多数其他遗传系统一样，植物线粒体中需要几个酶促加工和修饰步骤才能从初级转录本产生成熟的tRNA。其中涉及的三种酶，即核糖核酸酶P、3'-加工活性酶和tRNA核苷酸转移酶，已在马铃薯（Solanum tuberosum）线粒体中得到鉴定，并经过了几个纯化步骤进行分离。核糖核酸酶P经过了部分纯化，在最后的甘油梯度步骤后，活性组分中只能检测到少数几种蛋白质。具有核糖核酸酶P活性的组分中存在的一个小RNA分子含有在其他已知核糖核酸酶P RNA序列中保守的七核苷酸，可能是植物线粒体核糖核酸酶P的RNA部分的一个片段。
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