[Restriction digestion of two kind of baculoviruses and the location of their p10 genes].

The length of SeNPV and LsNPV genomes DNA were calculated to be 133kb and 164kb respectively, according to digestion with 5-8 restriction endonuleases. In order to map p10 gene in Baculoviruses DNA by pure probe, two probe vectors, pAcHP106 and pAcEP102, were constructed with 3'-0. 18kb and 3'-0. 18kb + 5'-0.24kb (coding sequence and promoter) from AcNPV p10 gene. The p10 genes of both baculoviruses, SeNPV and LsNPV, are located with 0.18kb and 0.42kb probes produced by random primer labeling.