Huang Y, Qi Y, Li L, Jin T
Department of Virology, Wuhan University.
Wei Sheng Wu Xue Bao. 1994 Jun;34(3):191-7.
The length of SeNPV and LsNPV genomes DNA were calculated to be 133kb and 164kb respectively, according to digestion with 5-8 restriction endonuleases. In order to map p10 gene in Baculoviruses DNA by pure probe, two probe vectors, pAcHP106 and pAcEP102, were constructed with 3'-0. 18kb and 3'-0. 18kb + 5'-0.24kb (coding sequence and promoter) from AcNPV p10 gene. The p10 genes of both baculoviruses, SeNPV and LsNPV, are located with 0.18kb and 0.42kb probes produced by random primer labeling.
